Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK.
Lab Chip. 2011 Dec 21;11(24):4267-73. doi: 10.1039/c0lc00357c. Epub 2011 Oct 27.
We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200 μm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics.
我们提出了一种简单的设备，用于多重定量酶联免疫吸附测定（ELISA），该设备由一种新型的熔融挤出微毛细管膜（MCF）制成，其中包含沿着其长度排列的 200μm 毛细管的平行阵列。为了制作 ELISA 设备，不同的蛋白质抗原或抗体被固定在从氟化乙烯丙烯（FEP）挤出的 MCF 的长卷中的各个微毛细管内。将涂覆的薄膜切成小块，并与移液器接口，允许从试剂孔中顺序将样品和检测溶液吸入所有毛细管中。这些 FEP MCF 设备不仅易于生产，而且具有出色的透光率，允许直接对毛细管进行光学检测，从而实现简单的信号定量。概念验证实验使用标准的直接 ELISA 程序在 FEP MCF 设备中证明了定量和多重检测，并使用平板扫描仪进行读取。这种新的多重免疫分析平台应该会在实验室检测、即时护理和现场诊断等领域找到应用。
