N-乙酰几丁糖对酿酒酵母几丁质生物合成的激活作用取决于壳寡糖的大小和结构。
Saccharomyces cerevisiae chitin biosynthesis activation by N-acetylchitooses depends on size and structure of chito-oligosaccharides.
作者信息
Becker Hubert F, Piffeteau Annie, Thellend Annie
机构信息
Laboratoire d'Optique et Biosciences, INSERM U696, CNRS UMR7645, Ecole Polytechnique, 91128 Palaiseau, France.
出版信息
BMC Res Notes. 2011 Oct 27;4:454. doi: 10.1186/1756-0500-4-454.
BACKGROUND
To explore chitin synthesis initiation, the effect of addition of exogenous oligosaccharides on in vitro chitin synthesis was studied. Oligosaccharides of various natures and lengths were added to a chitin synthase assay performed on a Saccharomyces cerevisiae membrane fraction.
FINDINGS
N-acetylchito-tetra, -penta and -octaoses resulted in 11 to 25% [14C]-GlcNAc incorporation into [14C]-chitin, corresponding to an increase in the initial velocity. The activation appeared specific to N-acetylchitooses as it was not observed with oligosaccharides in other series, such as beta-(1,4), beta-(1,3) or alpha-(1,6) glucooligosaccharides.
CONCLUSIONS
The effect induced by the N-acetylchitooses was a saturable phenomenon and did not interfere with free GlcNAc and trypsin which are two known activators of yeast chitin synthase activity in vitro. The magnitude of the activation was dependent on both oligosaccharide concentration and oligosaccharide size.
背景
为探究几丁质合成起始过程，研究了添加外源寡糖对体外几丁质合成的影响。将各种性质和长度的寡糖添加到对酿酒酵母膜组分进行的几丁质合酶测定中。
研究结果
N-乙酰壳四糖、N-乙酰壳五糖和N-乙酰壳八糖使[14C]-葡萄糖胺掺入[14C]-几丁质的量增加了11%至25%，这对应于初始速度的提高。这种激活似乎对N-乙酰壳寡糖具有特异性，因为在其他系列的寡糖中未观察到这种现象，例如β-(1,4)、β-(1,3)或α-(1,6)葡萄糖寡糖。
结论
N-乙酰壳寡糖诱导的效应是一种饱和现象，并且不干扰游离葡萄糖胺和胰蛋白酶，这两种物质是体外酵母几丁质合酶活性的已知激活剂。激活的程度取决于寡糖浓度和寡糖大小。
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