Laboratory of Genetics, Butantan Institute, Sao Paulo, Brazil.
Tissue Eng Part B Rev. 2012 Apr;18(2):129-38. doi: 10.1089/ten.TEB.2011.0327. Epub 2011 Dec 28.
Dental pulp from deciduous (baby) teeth, which are discarded after exfoliation, represents an advantageous source of young stem cells. Herein, we discuss the methods of deciduous teeth stem cell (DTSC) isolation and cultivation. We show that based on these methods, at least three different stem cell populations can be identified: a population similar to bone marrow-derived mesenchymal stem cells, an epithelial stem-like cells, and/or a mixed population composed of both cell types. We analyzed the embryonic origin and stem cell niche of DTSCs with respect to the advantages they can provide for their future use in cell therapies and regenerative medicine. In vitro and in vivo differentiation of the DTSC populations, their developmental potential, immunological compatibility, tissue engineering, and transplantation use in studies in animal models are also the focus of the current report. We briefly describe the derivation of induced pluripotent stem (iPS) cells from DTSCs, which can be obtained more easily and efficiently in comparison with human fibroblasts. These iPS cells represent an interesting model for the investigation of pediatric diseases and disorders. The importance of DTSC banking is also discussed.
脱落的乳牙（婴儿牙）牙髓是年轻干细胞的有利来源。本文讨论了乳牙干细胞（DTSC）分离和培养的方法。结果表明，基于这些方法，可以鉴定出至少三种不同的干细胞群体：类似于骨髓间充质干细胞的群体、上皮干细胞样细胞，和/或由这两种细胞类型组成的混合群体。我们分析了 DTSC 的胚胎起源和干细胞龛，以探讨其在细胞治疗和再生医学中的未来应用优势。DTSC 群体的体外和体内分化、其发育潜能、免疫相容性、组织工程以及在动物模型中的移植应用也是本报告的重点。我们简要描述了 DTSC 诱导多能干细胞（iPS）的衍生，与人类成纤维细胞相比，这更容易且更高效。这些 iPS 细胞为研究儿科疾病和障碍提供了一个有趣的模型。还讨论了 DTSC 库的重要性。
