Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1685, USA.
Insect Mol Biol. 2012 Feb;21(1):61-77. doi: 10.1111/j.1365-2583.2011.01113.x. Epub 2011 Oct 27.
Changes in gene expression before, during and after five generations of permethrin laboratory selection were monitored in six strains of Aedes aegypti: five F(2)-F(3) collections from the Yucatán Peninsula of Mexico and one F(2) from Iquitos, Peru. Three biological replicate lines were generated for each strain. The response to selection was measured as changes in the lethal and knockdown permethrin concentrations (LC(50), KC(50)) and in the frequency of the Ile1,016 substitution in the voltage-gated sodium channel (para) gene. Changes in expression of 290 metabolic detoxification genes were measured using the 'Aedes Detox' microarray. Selection simultaneously increased the LC(50), KC(50) and Ile1,016 frequency. There was an inverse relationship between Ile1,016 frequency and the numbers of differentially transcribed genes. The Iquitos strain lacked the Ile1,016 allele and 51 genes were differentially transcribed after selection as compared with 10-18 genes in the Mexican strains. Very few of the same genes were differentially transcribed among field strains but 10 cytochrome P(450) genes were upregulated in more than one strain. Laboratory adaptation to permethrin in Ae. aegypti is genetically complex and largely conditioned by geographic origin and pre-existing target site insensitivity in the para gene. The lack of uniformity in the genes that responded to artificial selection as well as differences in the direction of their responses challenges the assumption that one or a few genes control permethrin metabolic resistance. Attempts to identify one or a few metabolic genes that are predictably associated with permethrin adaptation may be futile.
在实验室中对拟除虫菊酯进行五代选择前后，监测了六种埃及伊蚊（Aedes aegypti）品系的基因表达变化：其中五个来自墨西哥尤卡坦半岛的 F(2)-F(3) 种群，一个来自秘鲁伊基托斯的 F(2) 种群。每个品系生成了三个生物学重复品系。选择的反应是通过测定致死和击倒拟除虫菊酯浓度（LC(50)，KC(50)）以及电压门控钠离子通道（para）基因中的 Ile1,016 取代的频率来衡量的。使用“埃及伊蚊解毒”微阵列测量了 290 种代谢解毒基因的表达变化。选择同时增加了 LC(50)、KC(50)和 Ile1,016 频率。Ile1,016 频率与差异转录基因的数量呈反比关系。伊基托斯品系缺乏 Ile1,016 等位基因，与墨西哥品系的 10-18 个基因相比，选择后有 51 个基因差异转录。田间种群中差异转录的基因很少相同，但 10 个细胞色素 P(450)基因在一个以上的种群中上调。埃及伊蚊对拟除虫菊酯的实验室适应是遗传复杂的，主要取决于地理起源和 para 基因中预先存在的靶标部位不敏感性。对人工选择有反应的基因缺乏一致性，以及它们的反应方向的差异，对控制拟除虫菊酯代谢抗性的一个或几个基因的假设提出了挑战。试图确定一个或几个与拟除虫菊酯适应可预测相关的代谢基因可能是徒劳的。
