Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, Haryana 125001, India.
Neurochem Res. 2012 Mar;37(3):487-94. doi: 10.1007/s11064-011-0635-7. Epub 2011 Oct 28.
The aim of present study was to evaluate antianxiety-like activity of gallic acid in Swiss young male albino mice; and to explore the possible underlying mechanisms for this activity. Gallic acid (5, 10, 20 mg/kg, i.p.) and alprazolam (0.25 mg/kg, i.p.) were administered for 10 successive days to separate groups of mice. On 10th day, 45 min after the drug administration, stress was produced by immobilization of mice for 150 min and these mice were called as stressed mice. Anxiolytic activity was evaluated using elevated plus maze and light-dark test. The plasma nitrite and corticosterone levels were also estimated in unstressed and stressed mice. Effects of 7-nitroindazole (neuronal NOS inhibitor) and aminoguanidine (inducible NOS inhibitor) on antianxiety-like activity of gallic acid were also evaluated. Gallic acid (10 and 20 mg/kg) and alprazolam per se significantly showed antianxiety-like activity in both unstressed and stressed mice. The drugs did not show any significant effect on locomotor activity of the mice. Gallic acid significantly decreased the plasma nitrite levels in both unstressed and stressed mice. 7-nitroindazole and aminoguanidine significantly enhanced antianxiety-like activity and plasma nitrite decreasing effect of gallic acid in unstressed and stressed mice respectively. Plasma corticosterone levels were significantly decreased by gallic acid in stressed mice as compared to its control. Thus, gallic acid showed antianxiety-like activity in unstressed mice probably by inhibition of nNOS. On the other hand, antianxiety-like activity in stressed mice might be through inhibition of iNOS and reduction of plasma corticosterone levels.
本研究旨在评估没食子酸在瑞士年轻雄性白化小鼠中的抗焦虑样活性；并探讨其活性的可能潜在机制。将没食子酸（5、10、20mg/kg，ip）和阿普唑仑（0.25mg/kg，ip）分别给予不同组别的小鼠连续 10 天。在第 10 天，给药 45 分钟后，通过将小鼠固定 150 分钟来产生应激，这些小鼠被称为应激小鼠。使用高架十字迷宫和明暗箱测试来评估抗焦虑活性。还在未应激和应激的小鼠中估计血浆亚硝酸盐和皮质酮水平。还评估了 7-硝基吲唑（神经元 NOS 抑制剂）和氨基胍（诱导型 NOS 抑制剂）对没食子酸抗焦虑样活性的影响。没食子酸（10 和 20mg/kg）和阿普唑仑本身在未应激和应激的小鼠中均显著表现出抗焦虑样活性。这些药物对小鼠的运动活动没有显示出任何显著影响。没食子酸显著降低了未应激和应激小鼠的血浆亚硝酸盐水平。7-硝基吲唑和氨基胍分别显著增强了未应激和应激小鼠中没食子酸的抗焦虑样活性和降低血浆亚硝酸盐的作用。与对照组相比，应激小鼠的血浆皮质酮水平显著降低。因此，没食子酸在未应激小鼠中表现出抗焦虑样活性，可能是通过抑制 nNOS。另一方面，应激小鼠中的抗焦虑样活性可能是通过抑制 iNOS 和降低血浆皮质酮水平来实现的。
