Uzawa S, Samejima I, Hirano T, Tanaka K, Yanagida M
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Cell. 1990 Sep 7;62(5):913-25. doi: 10.1016/0092-8674(90)90266-h.
Mutations in the fission yeast cut1+, cut2+, and cut10+ genes uncouple normally coordinated mitotic events and deregulate, rather than arrest, mitosis. DNA synthesis continues, making polyploid nuclei with several spindles. Multiple, aberrant spindle pole bodies (SPBs) are produced in cut1 mutant cells. The cut1+ and cut2+ genes are cloned by transformation. High gene dosage of cut1+ also complements cut2 and cut10 mutants. The cut2+ gene, however, complements only cut2. The 210 kd cut1+ gene product contains putative ATP binding and helical coil regions followed by a COOH-terminal domain homologous to the S. cerevisiae gene ESP1. Mutations in the ESP1 gene also result in many SPBs. The cut1+ product is shown by anti-cut1 antibody to be a rare component of the insoluble nuclear fraction. It may play a key role in coupling chromosome disjunction with other cell cycle events and is potentially a component, regulator, or motor for the SPB and/or kinetochores.
裂殖酵母cut1 +、cut2 +和cut10 +基因的突变会使正常协调的有丝分裂事件解偶联，并使有丝分裂失调而非停滞。DNA合成继续进行，形成具有多个纺锤体的多倍体细胞核。在cut1突变细胞中会产生多个异常的纺锤体极体（SPB）。通过转化克隆了cut1 +和cut2 +基因。cut1 +的高基因剂量也能互补cut2和cut10突变体。然而，cut2 +基因只能互补cut2。210 kd的cut1 +基因产物包含推定的ATP结合和螺旋卷曲区域，随后是与酿酒酵母基因ESP1同源的COOH末端结构域。ESP1基因的突变也会导致许多SPB。抗cut1抗体显示cut1 +产物是不溶性核部分的稀有成分。它可能在将染色体分离与其他细胞周期事件偶联中起关键作用，并且可能是SPB和/或动粒的组成部分、调节剂或动力。
