Department of Chemistry, The University of Michigan, Ann Arbor, Michigan, USA.
Nat Methods. 2011 Oct 28;8(11):919-31. doi: 10.1038/nmeth.1735.
Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural, kinetic and thermodynamic characteristics of RNA motions spanning picosecond to second timescales at atomic resolution, allowing unprecedented insights into the RNA dynamic structure landscape. From these studies a basic description of the RNA dynamic structure landscape is emerging, bringing new insights into how RNA structures change to carry out their function as well as applications in RNA-targeted drug discovery and RNA bioengineering.
许多最近发现的非编码 RNA 不会折叠成单一的天然构象，而是沿着它们的自由能景观采样许多不同的构象，以执行它们的生物学功能。在这里，我们回顾了溶液 NMR 技术，这些技术可以在原子分辨率下测量跨越皮秒到秒时间尺度的 RNA 运动的结构、动力学和热力学特性，从而以前所未有的方式深入了解 RNA 动态结构景观。从这些研究中，一个关于 RNA 动态结构景观的基本描述正在出现，这为我们提供了新的见解，了解 RNA 结构如何改变以执行其功能，以及在 RNA 靶向药物发现和 RNA 生物工程中的应用。
