Tordini Giacinta, Puttini Camilla, Rossetti Barbara, Sammarro Gabriella, Fanetti Alessandra, Cianchino Sergio, Valoriani Beatrice, Fossombroni Vittorio, Campoccia Giuseppe, Cavion Maria Antonietta, Zanelli Giacomo
Sezione Malattie Infettive Universitarie, Dipartimento Biotecnologie, Universita di Siena, Azienda Ospedaliera Universitaria Senese, Siena, Italy.
Infez Med. 2011 Sep;19(3):152-6.
Leishmaniasis is a protozoan infection endemic in Italy with a greatly underestimated prevalence. The recent documentation of parasitaemia in blood donors is a cause of concern for blood safety. Because there is no screening against leishmania, we performed a study to assess the presence of protozoa in blood donors of Siena district (Tuscany) during the seasonal activity of the vector. From June to October 2007, 162 patients were screened for Leishmania infantum by indirect immunofluorescence serology (IFAT) and PCR for kinetoplast (kDNA). No subject was positive for antibodies, while 11 samples (6.8%) were positive for kDNA. A second PCR (nested-PCR) was negative for all kDNA positive individuals and other subjects for a total of 55 samples (33% of total subjects). The sequence analysis of three samples positive for kDNA was compatible with mitochondrial DNA. Through the techniques used, we were unable to confirm the presence of leishmania in the blood of the subjects studied. The choice of the diagnostic protocol in blood donors remains an open issue as molecular analysis (kDNA) seems to suggest, in our experience, limits of specificity.
利什曼病是一种在意大利流行的原生动物感染病，其患病率被大大低估。近期关于献血者血中寄生虫血症的记录引发了对血液安全的担忧。由于没有针对利什曼原虫的筛查，我们开展了一项研究，以评估在媒介季节性活动期间锡耶纳地区（托斯卡纳）献血者中是否存在原生动物。2007年6月至10月，通过间接免疫荧光血清学（IFAT）和动基体（kDNA）的聚合酶链反应（PCR）对162名患者进行了婴儿利什曼原虫筛查。没有受试者抗体呈阳性，而11份样本（6.8%）kDNA呈阳性。对所有kDNA阳性个体以及另外总共55份样本（占总受试者的33%）进行的第二次PCR（巢式PCR）结果均为阴性。对3份kDNA阳性样本的序列分析与线粒体DNA相符。通过所使用的技术，我们无法证实所研究受试者血液中存在利什曼原虫。在献血者中选择诊断方案仍然是一个悬而未决的问题，因为根据我们的经验，分子分析（kDNA）似乎显示出特异性方面的局限性。
