Bioengineering Department, Yildiz Technical University, Istanbul, Turkey.
Am J Trop Med Hyg. 2012 Jul;87(1):81-6. doi: 10.4269/ajtmh.2012.12-0034.
The sensitivity of diagnostic methods for visceral leishmaniasis (VL) decreases because of the low number of parasites and antibody amounts in asymptomatic healthy donors who are not suitable for invasive sample acquisition procedures. Therefore, new studies are urgently needed to improve the sensitivity and specificity of the diagnostic approaches in non-invasive samples. In this study, the sensitivity of the microculture method (MCM) was compared with polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and immunofluorescent antibody test (IFAT) methods in an experimental murine model with asymptomatic leishmaniasis. Results showed that the percent of positive samples in ELISA, IFAT, and peripheral blood (PB) -PCR tests were 17.64%, 8.82%, and 5.88%, respectively, whereas 100% positive results were obtained with MCM and MCM-PCR methods. Thus, this study, for the first time, showed that MCM is more sensitive, specific, and economic than other methods, and the sensitivity of PCR that was performed to samples obtained from MCM was higher than sensitivity of the PCR method sampled by PB.
由于无症状健康供体中寄生虫数量和抗体量低，且不适合进行侵入性样本采集程序，因此用于内脏利什曼病（VL）的诊断方法的灵敏度会降低。因此，迫切需要开展新的研究来提高非侵入性样本中诊断方法的灵敏度和特异性。在本研究中，在无症状利什曼病的实验性小鼠模型中，比较了微培养法（MCM）与聚合酶链反应（PCR）、酶联免疫吸附测定（ELISA）和免疫荧光抗体试验（IFAT）方法的灵敏度。结果表明，ELISA、IFAT 和外周血（PB）-PCR 检测中的阳性样本百分比分别为 17.64%、8.82%和 5.88%，而 MCM 和 MCM-PCR 方法则获得了 100%的阳性结果。因此，本研究首次表明，MCM 比其他方法更敏感、更特异、更经济，且从 MCM 获得的样本进行的 PCR 比从 PB 进行的 PCR 方法的灵敏度更高。
