Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
PLoS One. 2011;6(10):e26880. doi: 10.1371/journal.pone.0026880. Epub 2011 Oct 24.
Campylobacter jejuni is the major cause of human bacterial gastroenteritis worldwide, and in a minority of cases, post-infectious complications may occur. ST-22 complex (usually Penner serotype 19) strains have been overrepresented among patients with postinfectious complications of campylobacteriosis. We here present a characterization of a collection of 27 Finnish C. jejuni strains of ST-22 complex, from humans (22 strains) and animal sources (five strains), with the aim of contributing to our knowledge of the pathogenesis of C. jejuni infections.
METHODOLOGY/PRINCIPAL FINDINGS: All strains were analyzed by pulsed-field gel electrophoresis (PFGE) genotyping, lipo-oligosaccharide (LOS) locus class, Y-glutamyl transpeptidase (GGT) activity, in vitro biofilm formation ability, invasion and adhesion in HeLa cells and induction of IL-8 production. ST-22 complex contained five STs (ST-22; ST-1947; ST-1966; ST-3892; ST-3996) which were homogeneous in having sialylated LOS class A(1) but on the other hand were distinguished into two major lineages according to the major STs (ST-22 and ST-1947) by different PFGE genotypes and certain other characteristics. All ST-22 strains had similar SmaI PFGE profiles, were GGT positive, and formed biofilms, except one strain, while ST-1947 strains were all GGT negative, did not form biofilm, had significantly higher motility than ST-22 (p<0.05) and had their SmaI PFGE profile. Invasion and adhesion as well as induction of IL-8 production on HeLa cells were strain-dependent characteristics.
CONCLUSIONS/SIGNIFICANCE: ST-22 complex strains, reveal potential for molecular mimicry in host interactions upon infection as they all express sialylated LOS class A(1). The two major STs, ST-22 and ST-1947 formed two homogeneous lineages, which differed from each other both phenotypically and genetically, suggesting that the strains may have evolved separately, perhaps by interacting with different spectra of hosts. Further studies are needed in order to understand if these two lineages are associated with different disease outcomes.
空肠弯曲菌是全世界人类细菌性胃肠炎的主要原因，在少数情况下，可能会发生感染后并发症。ST-22 复合群（通常为 Penner 血清型 19）菌株在弯曲菌病感染后并发症患者中过度表达。我们在此介绍了对来自人类（22 株）和动物源（5 株）的 27 株芬兰空肠弯曲菌 ST-22 复合群菌株的收集进行的特征描述，旨在增进我们对空肠弯曲菌感染发病机制的了解。
方法/主要发现：所有菌株均通过脉冲场凝胶电泳（PFGE）基因分型、脂寡糖（LOS）基因座类、γ-谷氨酰转肽酶（GGT）活性、体外生物膜形成能力、HeLa 细胞侵袭和黏附以及诱导 IL-8 产生进行分析。ST-22 复合群包含 5 种 ST（ST-22；ST-1947；ST-1966；ST-3892；ST-3996），它们的 LOS 类 A（1）均为唾液酸化，但另一方面，根据主要 ST（ST-22 和 ST-1947）的不同 PFGE 基因型和某些其他特征，它们分为两个主要谱系。所有 ST-22 菌株的 SmaI PFGE 图谱相似，GGT 阳性，形成生物膜，除了一株菌株外，而 ST-1947 菌株均为 GGT 阴性，不形成生物膜，运动性明显高于 ST-22（p<0.05），且 SmaI PFGE 图谱不同。侵袭和黏附以及在 HeLa 细胞上诱导 IL-8 产生均为菌株依赖性特征。
结论/意义：ST-22 复合群菌株在感染时表现出潜在的分子模拟宿主相互作用，因为它们都表达唾液酸化的 LOS 类 A（1）。两种主要 ST（ST-22 和 ST-1947）形成两个同质谱系，在表型和遗传上彼此不同，这表明这些菌株可能是通过与不同宿主谱相互作用而分别进化的。需要进一步研究才能了解这两个谱系是否与不同的疾病结果相关。
