Llarena Ann-Katrin, Huneau Adeline, Hakkinen Marjaana, Hänninen Marja-Liisa
Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
Anses, Ploufragan-Plouzané laboratory, BP 53, 22440, Ploufragan, France.
PLoS One. 2015 Feb 20;10(2):e0116585. doi: 10.1371/journal.pone.0116585. eCollection 2015.
Consumption and handling of chicken meat are well-known risk factors for acquiring campylobacteriosis. This study aimed to describe the Campylobacter jejuni population in Finnish chickens and to investigate the distribution of C. jejuni genotypes on Finnish chicken farms over a period of several years. We included 89.8% of the total C. jejuni population recovered in Finnish poultry during 2004, 2006, 2007, 2008, and 2012 and used multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) to characterize the 380 isolates. The typing data was combined with isolate information on collection-time and farm of origin. The C. jejuni prevalence in chicken slaughter batches was low (mean 3.0%, CI95% [1.8%, 4.2%]), and approximately a quarter of Finnish chicken farms delivered at least one positive chicken batch yearly. In general, the C. jejuni population was diverse as represented by a total of 63 sequence types (ST), but certain predominant MLST lineages were identified. ST-45 clonal complex (CC) accounted for 53% of the isolates while ST-21 CC and ST-677 CC covered 11% and 9% of the isolates, respectively. Less than half of the Campylobacter positive farms (40.3%) delivered C. jejuni-contaminated batches in multiple years, but the genotypes (ST and PFGE types) generally varied from year to year. Therefore, no evidence for a persistent C. jejuni source for the colonization of Finnish chickens emerged. Finnish chicken farms are infrequently contaminated with C. jejuni compared to other European Union (EU) countries, making Finland a valuable model for further epidemiological studies of the C. jejuni in poultry flocks.
食用和处理鸡肉是已知的感染弯曲菌病的风险因素。本研究旨在描述芬兰鸡肉中分离出的空肠弯曲菌菌群，并调查多年来芬兰养鸡场中空肠弯曲菌基因型的分布情况。我们纳入了2004年、2006年、2007年、2008年和2012年芬兰家禽中分离出的89.8%的空肠弯曲菌菌群，并用多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）对380株分离株进行了特征分析。分型数据与分离株的采集时间和来源农场信息相结合。鸡屠宰批次中空肠弯曲菌的患病率较低（平均3.0%，95%置信区间[1.8%，4.2%]），约四分之一的芬兰养鸡场每年至少交付一批阳性鸡。总体而言，空肠弯曲菌菌群具有多样性，共有63种序列类型（ST），但也确定了某些主要的MLST谱系。ST-45克隆复合体（CC）占分离株的53%，而ST-21 CC和ST-677 CC分别占分离株的11%和9%。不到一半的弯曲菌阳性农场（40.3%）在多年内交付了受空肠弯曲菌污染的批次，但基因型（ST和PFGE类型）通常逐年变化。因此，没有证据表明芬兰鸡的定植存在持续的空肠弯曲菌来源。与其他欧盟国家相比，芬兰养鸡场很少被空肠弯曲菌污染，这使得芬兰成为家禽群中空肠弯曲菌进一步流行病学研究的宝贵模型。
