[Toll样受体2和Toll样受体9基因多态性与胃癌易感性的相关性]

[The correlation between polymorphisms of Toll-like receptor 2 and Toll-like receptor 9 and susceptibility to gastric cancer].

作者信息

Zeng Hong-Mei, Pan Kai-Feng, Zhang Yang, Zhang Lian, Ma Jun-Ling, Zhou Tong, Su Hui-Juan, Li Wen-Qing, Li Ji-You, You Wei-Cheng

机构信息

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Cancer Epidemiology, Peking University Cancer Hospital & Institute, Beijing 100142, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2011 Jul;45(7):588-92.

PMID:22041559

Abstract

OBJECTIVE

To explore the relationship between the polymorphisms of Toll-like receptor 2 (TLR2) and TLR9 and the susceptibility to gastric cancer.

METHODS

A population-based case-control study was conducted at Linqu county, Shandong province, China, including a total of 248 cases of gastric cancer. Another total of 496 age and sex-matched controls were randomly selected from the same cohorts. TLR2 rs3804099 and TLR9 rs187084 were detected by polymerase chain reaction-restriction fragment length polymorphism method. Odds ratios (ORs) and 95% confidence interval (CI) were computed from logistic regression models after adjusting for age, sex, Helicobacter pylori (H. pylori) infection and smoking status.

RESULTS

The frequencies of TT, TC and CC genotype on TLR2 rs3804099 in control group were 43.5% (216/496), 46.6% (231/496) and 9.9% (49/496), respectively; whereas those in case group were 53.2% (132/248), 39.9% (99/248) and 6.9% (17/248), respectively. Significant differences in the frequencies of TLR2 rs3804099 were found between case and control groups (χ(2) = 6.665, P = 0.036). It was found that compared with the TT genotype, TC + CC genotype carriers obviously less susceptible to gastric cancer (OR = 0.68, 95%CI: 0.50 - 0.93). Joint effects analysis indicated that the TLR2 rs3804099 TT genotype carriers and H.pylori infectors had higher susceptibility to gastric cancer(OR = 3.42, 95%CI: 2.16 - 5.42), compared with TC + CC genotype carriers and non-H.pylori infection group. The frequencies of TT, TC and CC genotype on TLR9 rs187084 in control group were 33.3% (165/496), 49.0% (243/496) and 17.7% (88/496), respectively; whereas those in case group were 35.9% (89/248), 50.0% (124/248) and 14.1% (35/248), respectively. No significant association with gastric cancer was observed for TLR9 rs187084 polymorphism (χ(2) = 1.684, P = 0.431).

CONCLUSION

Our findings indicate that TLR2 rs3804099 is closely associated with susceptibility to gastric cancer.

摘要

目的

探讨Toll样受体2（TLR2）和TLR9基因多态性与胃癌易感性之间的关系。

方法

在中国山东省临朐县开展一项基于人群的病例对照研究，共纳入248例胃癌患者。另外从同一队列中随机选取496例年龄和性别匹配的对照。采用聚合酶链反应-限制性片段长度多态性方法检测TLR2 rs3804099和TLR9 rs187084。在调整年龄、性别、幽门螺杆菌（H. pylori）感染和吸烟状况后，通过逻辑回归模型计算比值比（OR）和95%置信区间（CI）。

结果

对照组中TLR2 rs3804099的TT、TC和CC基因型频率分别为43.5%（216/496）、46.6%（231/496）和9.9%（49/496）；而病例组中分别为53.2%（132/248）、39.9%（99/248）和6.9%（17/248）。病例组和对照组之间TLR2 rs3804099的频率存在显著差异（χ(2)=6.665，P = 0.036）。发现与TT基因型相比，TC + CC基因型携带者明显不易患胃癌（OR = 0.68，95%CI：0.50 - 0.93）。联合效应分析表明，与TC + CC基因型携带者和非H.pylori感染组相比，TLR2 rs3804099 TT基因型携带者和H.pylori感染者患胃癌的易感性更高（OR = 3.42，95%CI：2.16 - 5.42）。对照组中TLR9 rs187084的TT、TC和CC基因型频率分别为33.3%（165/496）、49.0%（243/496）和17.7%（88/496）；而病例组中分别为35.9%（89/248）、50.0%（124/248）和14.1%（35/248）。未观察到TLR9 rs187084多态性与胃癌有显著关联（χ(2)=1.684，P = 0.431）。