Semlali Abdelhabib, Almutairi Mikhlid, Parine Narasimha Reddy, Al Amri Abdullah, Shaik Jilani P, Al Naeem Abdulrahman, Abdulla Ajaj Sana, Rouabhia Mahmoud, Alanazi Mohammad Saud
Department of Biochemistry.
Zoology Department, College of Science, King Saud University.
Onco Targets Ther. 2017 Apr 27;10:2325-2333. doi: 10.2147/OTT.S121618. eCollection 2017.
Breast cancer (BC) is the most common cause of cancer-related deaths among women in the Kingdom of Saudi Arabia. An association between the dysregulation of innate immunity, primarily the deregulation of Toll-like receptors (TLRs), and BC development was described a long time ago. Several studies have reported that BC risk factors appear to be related to the interaction between certain genes and exposure to various environmental factors. Here, we investigated the potential correlation of three TLR2 single-nucleotide polymorphisms (SNPs; rs3804100, rs4696480, and rs3804099) with the development of BC in female patients from Saudi Arabia. We collected 126 blood samples from women with BC and 146 blood samples from healthy women without any clinical signs of BC. The genotypic frequencies of TLR2 polymorphisms were assayed. Our results showed that the genotypic and allelic frequencies of TLR2 did not differ significantly between BC patients and healthy controls. However, the distributions of rs3804100 (1350 T/C) genotypes in BC groups were 1%, 19%, and 80% for CC, CT, and TT, respectively. In the control group, the (1350 T/C) genotype distributions were 3%, 18%, and 79% for CC, CT, and TT, respectively. The SNP homozygous "TT" genotype was not associated with the risk of developing BC in the BC patients compared with controls (odds ratio [OR], 4.5; confidence interval [CI], 0.49-41.02; =0.145). The TLR2 rs4696480 AA genotype was observed in 23% of BC patients compared to 18% of control individuals, the AT genotype was seen in 40% of BC patients and 46% of control individuals, and the TT genotype was observed in 37% of BC patients and 36% of normal controls. Our results did not show any difference in genotypic frequency between BC patients and normal controls for the TLR2 rs3804099 SNP; however, the (C) phenotypic frequency was 49% in BC patients and 53% in controls. The (T) phenotypic frequency was 51% and 47% in BC patients and normal patients, respectively. These findings indicate that there is no association between the TLR2 polymorphisms tested and BC susceptibility in the female population from the Kingdom of Saudi Arabia. We suggest using other TLR2 SNPs to investigate the possible relationship between innate immunity deregulation by disruption of TLR2 and potential BC development.
乳腺癌（BC）是沙特阿拉伯王国女性癌症相关死亡的最常见原因。很久以前就描述了先天免疫失调，主要是Toll样受体（TLR）失调与BC发生之间的关联。几项研究报告称，BC风险因素似乎与某些基因和接触各种环境因素之间的相互作用有关。在此，我们调查了三个TLR2单核苷酸多态性（SNP；rs3804100、rs4696480和rs3804099）与沙特阿拉伯女性患者BC发生之间的潜在相关性。我们收集了126份BC女性患者的血样和146份无任何BC临床症状的健康女性的血样。检测了TLR2多态性的基因型频率。我们的结果表明，BC患者和健康对照之间TLR2的基因型和等位基因频率没有显著差异。然而，BC组中rs3804100（1350 T/C）基因型的分布，CC、CT和TT分别为1%、19%和80%。在对照组中，CC、CT和TT的（1350 T/C）基因型分布分别为3%、18%和79%。与对照组相比，BC患者中SNP纯合“TT”基因型与发生BC的风险无关（优势比[OR]为4.5；置信区间[CI]为0.49 - 41.02；P = 0.145）。在23%的BC患者中观察到TLR2 rs4696480 AA基因型，而对照组为18%；在40%的BC患者和46%的对照组个体中观察到AT基因型；在37%的BC患者和36%的正常对照组中观察到TT基因型。我们的结果未显示BC患者和正常对照之间TLR2 rs3804099 SNP的基因型频率有任何差异；然而，BC患者中（C）表型频率为49%，对照组为53%。BC患者和正常患者中（T）表型频率分别为51%和47%。这些发现表明，在沙特阿拉伯女性人群中，所检测的TLR2多态性与BC易感性之间没有关联。我们建议使用其他TLR2 SNP来研究TLR2破坏导致的先天免疫失调与潜在BC发生之间的可能关系。
