伊拉克不育男性精浆铜、镁水平与氧化应激的相关性
Association between seminal plasma copper and magnesium levels with oxidative stress in iraqi infertile men.
作者信息
Abdul-Rasheed Omar F
机构信息
Department of Chemistry and Biochemistry, College of Medicine/ Al-Nahrain University, Al-Kadhimiya- PO. Box: 70027, Baghdad- Iraq.
出版信息
Oman Med J. 2010 Jul;25(3):168-72. doi: 10.5001/omj.2010.51.
OBJECTIVES
To study the association between copper, magnesium and malondialdehyde levels in seminal plasma of oligozoospermic, azoospermic in relation to normozoospermic men.
METHODS
The present study was conducted at the Chemistry and Biochemistry department, College of Medicine, Al-Nahrain University, Baghdad-Iraq during September 2007 to February 2008 after obtaining approval from the research and ethics committee and obtaining written consent, 78 infertile men (age range 33.01±4.20 years) were recruited at the institute of embryo research and infertility treatment, Al-Kadhimiya teaching hospital, Iraq and were categorized according to their seminal fluid parameters to oligozoospermia (n=43) and azoospermia (n=35). 41 fertile men (age range 30.29±2.30 years) were selected as controls. Seminal plasma copper and magnesium were measured by atomic absorption spectrophotometry. Malondialdehyde was measured calorimetrically using thiobarbituric acid assay which detects thiobarbituric acid reactive substances.
RESULTS
Seminal plasma copper level was decreased significantly (p=0.000) in the azoospermic group compared to the control group. Whereas, the level decreased non-significantly in the oligozoospermic group. Seminal plasma magnesium levels were decreased significantly (p=0.000) in all the infertility groups studied. On the other hand, malondialdehyde levels which is an end product of lipid peroxidation were significantly elevated (p=0.000) in all the infertility groups studied.
CONCLUSION
Copper and magnesium work in different ways in order to maintain normal environment for spermatozoa for normal fertilization to occur.
目的
研究少精子症、无精子症患者与正常精子症男性精浆中铜、镁水平及丙二醛水平之间的关联。
方法
本研究于2007年9月至2008年2月在伊拉克巴格达纳赫兰大学医学院化学与生物化学系进行。经研究与伦理委员会批准并获得书面同意后，在伊拉克卡迪米亚教学医院胚胎研究与不孕治疗研究所招募了78名不育男性（年龄范围33.01±4.20岁），并根据精液参数将其分为少精子症组（n = 43）和无精子症组（n = 35）。选取41名有生育能力的男性（年龄范围30.29±2.30岁）作为对照组。采用原子吸收分光光度法测定精浆铜和镁含量。使用检测硫代巴比妥酸反应性物质的硫代巴比妥酸法通过比色法测定丙二醛含量。
结果
与对照组相比，无精子症组精浆铜水平显著降低（p = 0.000）。而少精子症组该水平降低不显著。在所有研究的不育组中，精浆镁水平均显著降低（p = 0.000）。另一方面，作为脂质过氧化终产物的丙二醛水平在所有研究的不育组中均显著升高（p = 0.000）。
结论
铜和镁以不同方式发挥作用，以维持精子正常受精的正常环境。
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