Son Mike S, Taylor Ronald K
Dartmouth Medical School, Hanover, New Hampshire, USA.
Curr Protoc Microbiol. 2011 Nov;Chapter 6:Unit6A.3. doi: 10.1002/9780471729259.mc06a03s23.
The vibriocidal titer assay can be used to detect antibodies against Vibrio cholerae in serum samples, serving as an indicator of prior infection and potential protection against cholera. The assay can be utilized in research and clinical settings to test the effectiveness of vaccines, and also in epidemiological studies relevant to cholera transmission and surveillance. This unit outlines the steps involved in conducting an easily interpreted colorimetric vibriocidal titer assay with a relatively short turnaround time for results of around 8 hr, with final result observations in 24 hr. The assay can also be easily scaled up or down to accommodate as many or as few serum samples available and is not V. cholerae strain specific.
杀弧菌滴度测定可用于检测血清样本中针对霍乱弧菌的抗体，作为既往感染和对霍乱潜在保护作用的指标。该测定可用于研究和临床环境中以测试疫苗的有效性，也可用于与霍乱传播和监测相关的流行病学研究。本单元概述了进行一种易于解释的比色杀弧菌滴度测定的步骤，结果周转时间相对较短，约8小时，最终结果在24小时内观察。该测定也可轻松扩大或缩小规模，以适应可用血清样本的多少，且不具有霍乱弧菌菌株特异性。
