Topaloglu-Ak Asli, Ertugrul Fahinur, Eden Ece, Ates Mustafa, Bulut Hakan
Department of Pedodontics, Ege University School of Dentistry, Ismir, Turkey.
J Clin Pediatr Dent. 2011 Summer;35(4):433-6. doi: 10.17796/jcpd.35.4.61114412637mt661.
This study investigates the effect of fixed and removable orthodontic appliances among children on salivary Streptococcus mutans, Lactobacillus sp. and Candida albicans.
The study was conducted on 69 patients aged between 6-17 years who used fixed or removable orthodontic appliances. Five ml samples of unstimulated saliva from each patient were collected at baseline and at the 1, 3 and 6 month periodic controls. Samples were diluted and plated on Mitis Salivarus Agar (MSA), Man Rogosa Sharp Agar (MRS) and Saboroud Dextrose Agar (SDA). The growths on the plates were examined under a stereomicroscope. Numbers of colony forming units (CFU) per plate were counted. For statistical analysis, the paired t test and Chi-Square were used.
S mutans and Lactobacillus sp counts increased significantly 6 months after the insertion of fixed/removable orthodontic appliances in the oral cavity. A significant increase for C albicans presence was noted after 3 months compared with baseline for fixed appliances.
Long-term utilization of orthodontic appliances may have a negative effect on microbial flora and increase the risk of new carious lesions and periodontal problems. Patients should be recalled within short time intervals to be motivated for oral hygiene during their orthodontic therapy.
本研究调查儿童使用固定和活动正畸矫治器对唾液中变形链球菌、乳酸杆菌属和白色念珠菌的影响。
对69例年龄在6至17岁之间使用固定或活动正畸矫治器的患者进行研究。在基线以及第1、3和6个月定期复查时，从每位患者采集5毫升未刺激唾液样本。将样本稀释后接种于轻唾琼脂（MSA）、曼-罗氏-夏普琼脂（MRS）和沙氏葡萄糖琼脂（SDA）上。在体视显微镜下检查平板上的生长情况。计算每平板上的菌落形成单位（CFU）数量。采用配对t检验和卡方检验进行统计分析。
在口腔内佩戴固定/活动正畸矫治器6个月后，变形链球菌和乳酸杆菌属数量显著增加。与基线相比，固定矫治器佩戴3个月后白色念珠菌的存在显著增加。
长期使用正畸矫治器可能对微生物菌群产生负面影响，并增加新发龋损和牙周问题的风险。在正畸治疗期间，应在短时间间隔内召回患者，以促使其保持口腔卫生。
