Kasibut Punnisa, Kuvatanasuchati Jintakorn, Thaweboon Boonyanit, Sirisoontorn Irin
Department of Clinical Dentistry, Walailak University International College of Dentistry (WUICD), 87 Ranong 2 Road, Dusit, Bangkok 10300, Thailand.
Department of Oral Health Science, Walailak University International College of Dentistry (WUICD), 87 Ranong 2 Road, Dusit, Bangkok 10300, Thailand.
Polymers (Basel). 2022 Aug 30;14(17):3583. doi: 10.3390/polym14173583.
The oral microbiome can be shifted if the patients wear the acrylic retainers for a lengthy period. It is essential to understand the components of the plaque in order to forestall the development of dental caries and gingivitis. The aim of this study is to report the bacterial communities that adhere to the acrylic retainers by full-length nanopore 16S sequencing. Six healthy participants were allocated into 2 groups (chemical tablet and brushing groups). Plaque samples were collected from the acrylic retainer surfaces before and after cleaning. The bacterial communities were reported using full-length nanopore 16S sequencing. The results showed that 7 distinct phyla were identified by sequencing. The most prevalent of these was the Firmicutes. We found a total of 72 genera. The most common microorganism across all samples was Streptococcus, followed by Neisseria, Rothia, and Gemella. The beta diversity showed a significant difference between before and after cleaning (p < 0.05). This study revealed the novel finding that a combination of chemical and mechanical cleaning methods was the most effective method of eliminating retainer biofilms. Moreover, retainer cleaning tablets did not alter the homeostatic balance of the bacterial communities adhering to the acrylic retainers.
如果患者长时间佩戴丙烯酸类保持器，口腔微生物群可能会发生变化。了解牙菌斑的成分对于预防龋齿和牙龈炎的发展至关重要。本研究的目的是通过全长纳米孔16S测序报告粘附在丙烯酸类保持器上的细菌群落。六名健康参与者被分为两组（化学片剂组和刷牙组）。在清洁前后从丙烯酸类保持器表面采集牙菌斑样本。使用全长纳米孔16S测序报告细菌群落。结果显示，测序鉴定出7个不同的门。其中最普遍的是厚壁菌门。我们总共发现了72个属。所有样本中最常见的微生物是链球菌，其次是奈瑟菌、罗氏菌和孪生球菌。β多样性显示清洁前后存在显著差异（p<0.05）。本研究揭示了一个新发现，即化学和机械清洁方法相结合是消除保持器生物膜最有效的方法。此外，保持器清洁片不会改变粘附在丙烯酸类保持器上的细菌群落的稳态平衡。
