Dipartimento di Scienze e Agrarie ed Ambientali, Università degli Studi di Udine, via delle Scienze 208, 33100 Udine, Italy.
Nano Lett. 2011 Dec 14;11(12):5449-54. doi: 10.1021/nl203217m. Epub 2011 Nov 4.
A DNA-origami actuator capable of autonomous internal motion in accord to an external chemical signal was designed, built, operated and imaged. The functional DNA nanostructure consists of a disk connected to an external ring in two, diametrically opposite points. A single stranded DNA, named probe, was connected to two edges of the disk perpendicularly to the axis of constrain. In the presence of a hybridizing target molecule, the probe coiled into a double helix that stretched the inner disk forcing the edges to move toward each other. The addition of a third single stranded molecule that displaced the target from the probe restored the initial state of the origami. Operation, dimension and shape were carefully characterized by combining microscopy and fluorescence techniques.
设计、构建、操作和成像了一种能够根据外部化学信号自主进行内部运动的 DNA 折纸致动器。该功能 DNA 纳米结构由一个圆盘和一个外部环组成，两个结构在两个直径相对的点连接。一条名为探针的单链 DNA 垂直于约束轴连接到圆盘的两条边缘。在存在杂交靶分子的情况下，探针盘绕成一个双螺旋，拉伸内盘，迫使边缘相互靠近。添加第三个单链分子，将靶分子从探针上置换下来，从而恢复折纸的初始状态。通过结合显微镜和荧光技术，仔细地对操作、尺寸和形状进行了表征。
