Danish National Research Foundation, Center for DNA, Nanotechnology, Department of Chemistry and iNANO, Gustav Wieds Vej 14, 8000, Aarhus C, Denmark.
Angew Chem Int Ed Engl. 2017 Oct 23;56(44):13633-13636. doi: 10.1002/anie.201706680. Epub 2017 Sep 27.
Signal transfer is central to the controlled exchange of information in biology and advanced technologies. Therefore, the development of reliable, long-range signal transfer systems for artificial nanoscale assemblies is of great scientific interest. We have designed such a system for the signal transfer between two connected DNA nanostructures, using the hybridization chain reaction (HCR). Two sets of metastable DNA hairpins, one of which is immobilized at specific points along tracks on DNA origami structures, are polymerized to form a continuous DNA duplex, which is visible using atomic force microscopy (AFM). Upon addition of a designed initiator, the initiation signal is efficiently transferred more than 200 nm from a specific location on one origami structure to an end point on another origami structure. The system shows no significant loss of signal when crossing from one nanostructure to another and, therefore, has the potential to be applied to larger multi-component DNA assemblies.
信号转导是生物学和先进技术中信息控制交换的核心。因此，开发用于人工纳米级组件的可靠、长程信号转导系统具有重要的科学意义。我们使用杂交链式反应 (HCR) 为两个连接的 DNA 纳米结构之间的信号转导设计了这样一个系统。两组亚稳态 DNA 发夹，其中一个固定在 DNA 折纸结构上的特定点沿线的轨道上，聚合形成连续的 DNA 双链体，可使用原子力显微镜 (AFM) 观察到。加入设计的引发剂后，起始信号可从一个折纸结构上的特定位置高效地转移超过 200nm 到另一个折纸结构上的端点。当从一个纳米结构到另一个纳米结构时，该系统不会明显损失信号，因此有可能应用于更大的多组分 DNA 组件。
