Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Molecules. 2011 Nov 2;16(11):9194-206. doi: 10.3390/molecules16119194.
The aim of the study was to investigate the effect of Ginkgo biloba extract 50 (GBE50), a well-known natural antioxidant, against immunity and antioxidant enzyme activities in ischemia reperfusion (IR) rats. Rats were then divided into six groups fed for 15 days with the same diet: three groups (IV, V, VI) were treated by different doses of GBE50 suspension [20, 40, or 60 mg/kg body weight by oral gavage every day at a fixed time (10.00 a.m.)] (equal to 5, 10 and 20 times, respectively, the maximum recommended human dose), and three groups (I, II, III) were untreated. At the end of the experiment, rats' hearts were subjected to 30 min of ischemia followed by 90 min of reperfusion. Results showed that IR significantly enhanced heart rate, S-T height, myocardium (myeloperoxidase) MPO activity and blood interleukin-8 (IL-8), tumor necrosis factor Alpha (TNF-a), interleukin-1β (IL-1β) levels, blood aspartate transaminase (AST), lactate dehydrogenase (LDH), and creatinine kinase (CK) activities, reduced myocardium sodium-potassium adenosine triphosphatase (Na(+)-K(+)-ATPase), calcium-magnesium adenosine triphosphatase (Ca(2+)-Mg(2+)-ATPase) activities and antioxidant enzyme activities in IR group (III) compared to sham control group (II). Pretreatment of GBE50 markedly significantly reduced heart rate, S-T height, myocardium MPO activity and blood IL-8, TNF-a, IL-1β levels, blood AST, LDH, and CK activities, enhanced myocardium Na(+)-K(+)-ATPase, Ca(2+)-Mg(2+)-ATPase activities and antioxidant enzyme activities in IR group (II) compared to IR group (III). The results suggested that the GBE50 may reduce the oxidative stress in the reperfused myocardium, and increased immunity and antioxidant activities in IR rats.
本研究旨在探讨银杏叶提取物 50（GBE50）作为一种著名的天然抗氧化剂，对缺血再灌注（IR）大鼠的免疫和抗氧化酶活性的影响。随后，将大鼠分为六组，用相同的饮食喂养 15 天：三组（IV、V、VI）通过口服给予不同剂量的 GBE50 混悬液[20、40 或 60mg/kg 体重，每天固定时间（上午 10 点）]（分别相当于最大推荐人用剂量的 5、10 和 20 倍），三组（I、II、III）未治疗。在实验结束时，大鼠的心脏经历 30 分钟的缺血，然后再灌注 90 分钟。结果表明，IR 显著增强了心率、S-T 高度、心肌（髓过氧化物酶）MPO 活性和血液白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）水平、血液天门冬氨酸氨基转移酶（AST）、乳酸脱氢酶（LDH）和肌酸激酶（CK）活性，降低了心肌钠钾三磷酸腺苷（Na(+)-K(+)-ATPase）、钙镁三磷酸腺苷（Ca(2+)-Mg(2+)-ATPase）活性和抗氧化酶活性，与假手术对照组（II）相比，IR 组（III）。GBE50 的预处理显著降低了心率、S-T 高度、心肌 MPO 活性和血液 IL-8、TNF-α、IL-1β 水平、血液 AST、LDH 和 CK 活性，增强了 IR 组（II）的心肌 Na(+)-K(+)-ATPase、Ca(2+)-Mg(2+)-ATPase 活性和抗氧化酶活性，与 IR 组（III）相比。结果表明，GBE50 可能减轻再灌注心肌的氧化应激，提高 IR 大鼠的免疫力和抗氧化活性。
