Department of Microbiology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
Biofouling. 2011 Nov;27(10):1087-93. doi: 10.1080/08927014.2011.631169.
Lytic bacteriophages (phages) have been investigated as treatments for bacterial infectious diseases. An induced phage, SAP-26, was isolated from a clinical isolate of Staphylococcus aureus. It belongs to the family Siphoviridae and its genome consists of double-stranded 41,207 bp DNA coding for 63 open reading frames. The phage SAP-26 showed a wide spectrum of lytic activity against both methicillin-resistant S. aureus and methicillin-susceptible S.aureus. Furthermore, combined treatment with a phage and antimicrobial agents showed a strong biofilm removal effect which induced structural changes in the biofilm matrix and a substantial decrease in the number of bacteria. Such a broad host range in S. aureus and biofilm removal activity of the phage SAP-26 suggests the possibility of its use as a therapeutic phage in combination with appropriate antimicrobial agent(s). Among the three antimicrobial agents combined with phage, the combination of rifampicin showed the best biofilm removal effect. To the authors' knowledge, this study showed for the first time that S. aureus biofilm could be efficiently eradicated with the mixture of phage and an antimicrobial agent, especially rifampicin.
溶菌噬菌体（噬菌体）已被研究用于治疗细菌性传染病。从金黄色葡萄球菌的临床分离株中分离出一种诱导噬菌体 SAP-26。它属于长尾噬菌体科，基因组由双链 41,207 bp DNA 组成，编码 63 个开放阅读框。噬菌体 SAP-26 对耐甲氧西林金黄色葡萄球菌和甲氧西林敏感金黄色葡萄球菌均表现出广泛的溶菌活性。此外，噬菌体与抗菌药物联合治疗具有很强的去除生物膜的效果，可诱导生物膜基质发生结构变化，并显著减少细菌数量。噬菌体 SAP-26 在金黄色葡萄球菌中具有广泛的宿主范围和去除生物膜的活性，这表明它有可能与适当的抗菌药物联合用作治疗性噬菌体。在与噬菌体联合使用的三种抗菌药物中，利福平的组合显示出最好的生物膜去除效果。据作者所知，这项研究首次表明，噬菌体和抗菌药物的混合物，尤其是利福平，可以有效地清除金黄色葡萄球菌生物膜。
