Department of Food and Animal Biotechnology, Seoul National University, Seoul 08826, Korea.
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.
Viruses. 2019 Jan 12;11(1):54. doi: 10.3390/v11010054.
Staphylococcus aureus is one of the notable human pathogens that can be easily encountered in both dietary and clinical surroundings. Among various countermeasures, bacteriophage therapy is recognized as an alternative method for resolving the issue of antibiotic resistance. In the current study, bacteriophage CSA13 was isolated from a chicken, and subsequently, its morphology, physiology, and genomics were characterized. This Podoviridae phage displayed an extended host inhibition effect of up to 23 hours of persistence. Its broad host spectrum included methicillin susceptible S. aureus (MSSA), methicillin resistant S. aureus (MRSA), local S. aureus isolates, as well as non-aureus staphylococci strains. Moreover, phage CSA13 could successfully remove over 78% and 93% of MSSA and MRSA biofilms in an experimental setting, respectively. Genomic analysis revealed a 17,034 bp chromosome containing 18 predicted open reading frames (ORFs) without tRNAs, representing a typical chromosomal structure of the staphylococcal Podoviridae family. The results presented here suggest that phage CSA13 can be applicable as an effective biocontrol agent against S. aureus.
金黄色葡萄球菌是一种重要的人类病原体，在饮食和临床环境中都很容易遇到。在各种对策中，噬菌体治疗被认为是解决抗生素耐药性问题的一种替代方法。在本研究中，从一只鸡中分离出了噬菌体 CSA13，并对其形态、生理和基因组进行了表征。这种 Podoviridae 噬菌体表现出长达 23 小时的持续宿主抑制效应。其广泛的宿主谱包括甲氧西林敏感金黄色葡萄球菌（MSSA）、甲氧西林耐药金黄色葡萄球菌（MRSA）、局部金黄色葡萄球菌分离株以及非金黄色葡萄球菌菌株。此外，噬菌体 CSA13 能够成功去除 MSSA 和 MRSA 生物膜的 78%以上和 93%以上，分别。基因组分析显示，该噬菌体含有 17034bp 的染色体，包含 18 个预测的开放阅读框（ORFs），不含 tRNAs，代表了葡萄球菌 Podoviridae 家族的典型染色体结构。本研究结果表明，噬菌体 CSA13 可用作金黄色葡萄球菌的有效生物防治剂。
