Department of Histology, Microbiology and Medical Biotechnologies, Division of Microbiology and Virology, University of Padova, Via A, Gabelli 63, Padova 35121, Italy.
Virol J. 2011 Nov 3;8:502. doi: 10.1186/1743-422X-8-502.
From October 2010 to April 2011, in the Italian Veneto Region, 1403 hospitalized patients were tested for influenza virus infection by specific real time RT-PCR. Overall, 327 samples were positive for either influenza A (75%) or B (25%) viruses. Among these positive patients two resulted co-infected by A/H1N1v and B viruses. Even though co-infection with both influenza A and B viruses appears to be a rare event, it occurs naturally and may play a role in epidemiology and pathogenicity. In the present study the two co-infected patients were a transplant recipient immunocompromised adult and a child displaying a severe respiratory illness. The co-infection was confirmed by inoculation of the nasopharyngeal swabs in MDCK.2 cells, followed by immunofluorescence and real time RT-PCR assays. Moreover, in the case of the adult patient, the immune system response against both viruses was assayed by hemoagglutination inhibition test against reference influenza virus strains. Both patients fully recovered from infection, without significant differences with mono-infected patients.
从 2010 年 10 月至 2011 年 4 月，意大利威尼托地区对 1403 名住院患者进行了流感病毒感染的特定实时 RT-PCR 检测。总体而言，327 个样本呈 A 型（75%）或 B 型（25%）流感病毒阳性。在这些阳性患者中，有两名同时感染了 A/H1N1v 和 B 型病毒。尽管同时感染 A 和 B 型流感病毒似乎是一种罕见的情况，但它是自然发生的，可能在流行病学和致病性方面发挥作用。在本研究中，两名同时感染的患者是一名免疫功能低下的成年移植受者和一名患有严重呼吸道疾病的儿童。通过将鼻咽拭子接种到 MDCK.2 细胞中，随后进行免疫荧光和实时 RT-PCR 检测，确认了这种合并感染。此外，对于成年患者，通过针对参考流感病毒株的血凝抑制试验测定了针对两种病毒的免疫系统反应。两名患者均从感染中完全康复，与单感染患者无明显差异。
