Jokipii L, Jokipii A M
Med Microbiol Immunol. 1979 Mar 13;167(1):61-70. doi: 10.1007/BF02123297.
The bioassay of metronidazole using Clostridium butyricum incorporated in agar plates detected concentrations higher than 1.0 microgram/ml. Gentamicin in the sample did not affect the growth of the target organism or the inhibition by metronidazole. Penicillin in the sample could be eliminated by the incorporation of penicillinase in the agar or by using as the target organism a surface inoculum of the penicillin-resistant Bacteroides fragilis. Increasing duration and temperature of aerobic prediffusion before allowing the growth of the strictly anaerobic Cl. butyricum increased the diameters of inhibition by metronidazole, but did not affect the threshold of detectability. The incorporation of metronidazole in the agar increased the sensitivity of the assay ten- to 100-fold.
使用掺入琼脂平板中的丁酸梭菌对甲硝唑进行生物测定，检测到浓度高于1.0微克/毫升。样品中的庆大霉素不影响目标微生物的生长或甲硝唑的抑制作用。样品中的青霉素可通过在琼脂中加入青霉素酶或使用耐青霉素的脆弱拟杆菌表面接种物作为目标微生物来消除。在允许严格厌氧的丁酸梭菌生长之前，增加有氧预扩散的持续时间和温度会增加甲硝唑的抑菌圈直径，但不影响可检测阈值。在琼脂中加入甲硝唑可使测定灵敏度提高10至100倍。
