Hochi Shinichi, Abdalla Hany, Hara Hiromasa, Hirabayashi Masumi
Graduate School of Science and Technology, Shinshu University, Nagano 386-8567, Japan.
J Reprod Dev. 2011 Oct;57(5):557-63. doi: 10.1262/jrd.11-061o.
Freeze-drying (lyophilization) has been proposed as an alternative method for sperm preservation to overcome the disadvantages of the current cryopreservation method such as the high maintenance cost of frozen stocks, the problems associated with transportation of frozen materials and the potential risk of total loss of the frozen stock. Since freeze-dried spermatozoa after rehydration lose their motility, which is an essential requirement to complete physiological fertilization, a relatively difficult microinsemination technique must be applied to rehydrated spermatozoa. Theoretically, it has been supposed that freeze-dried spermatozoa could maintain their functions and abilities to interact with the oocyte cytoplasm after prolonged storage at refrigerator temperature. However, sufficient yield of transferable blastocysts and production of live offspring derived from freeze-dried sperm samples are still subjects to be challenged and overcome in large domestic species.
冻干（冷冻干燥）已被提议作为一种精子保存的替代方法，以克服当前冷冻保存方法的缺点，如冷冻库存的高昂维护成本、与冷冻材料运输相关的问题以及冷冻库存完全损失的潜在风险。由于复水后的冻干精子会失去活力，而活力是完成生理受精的一项基本要求，因此必须对复水后的精子应用相对困难的显微授精技术。从理论上讲，人们认为冻干精子在冰箱温度下长期保存后仍能保持其功能以及与卵母细胞细胞质相互作用的能力。然而，在大型家畜物种中，来自冻干精子样本的可移植囊胚的充足产量和活体后代的产生仍有待挑战和克服。
