Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, PR China.
J Sep Sci. 2011 Nov;34(22):3240-5. doi: 10.1002/jssc.201100578. Epub 2011 Nov 3.
An extraction agent featuring a natural product, baicalin, anchored on the surface of nanomagnetic particles (BMNPs) is herein reported. A facile solid-phase extraction (SPE) procedure with high selectivity toward flavonoids using BMNPs has been established. BMNPs were proven very effective for enriching flavonoids from extracts of medicinal plants such as Rosa chinensis. The SPE protocol involving a convenient solid-liquid separation by using an external magnet field was easy to carry out. Further, the SPE sorbent (BMNPs) could be reused for many times reducing the operation cost. Importantly, flavonoids retained on the BMNPs were effectively recovered by eluting with methanol. Coupling the proposed SPE with ESI-MS/MS allowed a quick quantification of flavonoids in herbal extracts. Simultaneous determination of eight flavonoids extracted from R. chinensis was demonstrated in this work.
本文报道了一种以天然产物黄芩苷为配体锚定在纳米磁性粒子（BMNPs）表面的萃取剂。建立了一种使用 BMNPs 对黄酮类化合物具有高选择性的简便固相萃取（SPE）方法。BMNPs 被证明非常有效地从药用植物如玫瑰中提取黄酮类化合物。该 SPE 方案涉及通过使用外部磁场进行方便的固-液分离，易于实施。此外，SPE 吸附剂（BMNPs）可以重复使用多次，降低了操作成本。重要的是，用甲醇洗脱可以有效地回收保留在 BMNPs 上的黄酮类化合物。将所提出的 SPE 与 ESI-MS/MS 相结合，允许快速定量草药提取物中的黄酮类化合物。本工作中同时测定了从 R. chinensis 中提取的 8 种黄酮类化合物。
