Leung Ken Cham-Fai, Seneviratne Chaminda Jayampath, Li Xuan, Leung Ping Chung, Lau Clara Bik San, Wong Chi-Hin, Pang Ka Yan, Wong Chun Wai, Wat Elaine, Jin Lijian
Department of Chemistry, Institute of Creativity, and Partner State Key Laboratory of Environmental and Biological Analysis, The Hong Kong Baptist University, Kowloon, Hong Kong, China.
Faculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, Singapore City 119083, Singapore.
Nanomaterials (Basel). 2016 Apr 7;6(4):61. doi: 10.3390/nano6040061.
(SB) is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticle-encapsulated chlorhexidine (Nano-CHX). Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB (Nano-SB) and Nano-CHX on oral bacterial biofilms. Loading efficiency of Nano-SB was determined by thermogravimetric analysis, and its releasing profile was assessed by high-performance liquid chromatographyusing baicalin (a flavonoid compound of SB) as the marker. The mucosal diffusion assay on Nano-SB was undertaken in a porcine model. The antibacterial effects of the mixed nanoparticles (Nano-MIX) of Nano-SB and Nano-CHX at 9:1 (/) ratio were analyzed in both planktonic and biofilm modes of representative oral bacteria. The Nano-MIX was effective on the mono-species biofilms of () , , () , and () (MIC 50 μg/mL) at 24 h, and exhibited an enhanced effect against the multi-species biofilms such as , , , and () (MIC 12.5 μg/mL) at 24 h that was supported by the findings of both scanning electron microscopy (SEM) and confocal scanning laser microscopy (CLSM). This study shows enhanced synergistic antibacterial effects of the Nano-MIX on common oral bacterial biofilms, which could be potentially developed as a novel antimicrobial agent for clinical oral/periodontal care.
（SB）是一种用于治疗感染性和炎症性疾病的中药。我们最近的研究表明，纳米颗粒包裹的洗必泰（Nano-CHX）具有强大的抗菌作用。在此，我们探讨了纳米颗粒包裹的SB（Nano-SB）和Nano-CHX对口腔细菌生物膜的协同作用。通过热重分析测定Nano-SB的负载效率，并以黄芩苷（SB的一种黄酮类化合物）为标志物，采用高效液相色谱法评估其释放曲线。在猪模型中进行了Nano-SB的黏膜扩散试验。分析了Nano-SB和Nano-CHX以9:1（/）比例混合的纳米颗粒（Nano-MIX）对代表性口腔细菌的浮游菌和生物膜模式的抗菌作用。Nano-MIX在24小时时对牙龈卟啉单胞菌（Porphyromonas gingivalis）、具核梭杆菌（Fusobacterium nucleatum）、伴放线聚集杆菌（Aggregatibacter actinomycetemcomitans）和金黄色葡萄球菌（Staphylococcus aureus）的单菌种生物膜有效（MIC为50μg/mL），并且扫描电子显微镜（SEM）和共聚焦扫描激光显微镜（CLSM）的结果均表明，其在24小时时对多菌种生物膜如牙龈卟啉单胞菌、具核梭杆菌、中间普氏菌（Prevotella intermedia）和金黄色葡萄球菌（MIC为12.5μg/mL）具有增强的抗菌效果。本研究表明Nano-MIX对常见口腔细菌生物膜具有增强的协同抗菌作用，有望开发成为一种用于临床口腔/牙周护理的新型抗菌剂。
