BAC DNA 的高效转基因的纯化。
Purification of BAC DNA for high-efficiency transgenesis.
机构信息
Jules Stein Eye Institute, UCLA, University of California, Los Angeles, CA 90095-700019, USA.
出版信息
Biotechniques. 2011 Nov;51(5):335-6, 338. doi: 10.2144/000113763.
An unresolved bottleneck in bacterial artificial chromosome (BAC) transgenesis is low efficiency generation of founder mice because of suboptimal quality of the manipulated BAC DNA. Using mini-gel electrophoresis and electro-elution that circumvents CsCl(2) centrifugation, column chromatography, and resin purifications, we have used RECOCHIP, a commercially available dialysis cassette for the purification of BAC DNA that generates transgenic founders with up to 80% efficiency.
在细菌人工染色体 (BAC) 转基因中，一个未解决的瓶颈是由于操作 BAC DNA 的质量不理想，导致产生起始小鼠的效率低。通过使用迷你凝胶电泳和电洗脱绕过 CsCl(2) 离心、柱层析和树脂纯化，我们使用了 RECOCHIP，这是一种商业上可用于 BAC DNA 纯化的透析盒，可产生高达 80%效率的转基因起始小鼠。
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