Rochet N, Sadoul J L, Ferrua B, Kubar J, Tanti J F, Bougnères P, Vialettes B, Van Obberghen E, Le Marchand-Brustel Y, Freychet P
INSERM U 145, Faculté de Médecine, Nice, France.
Diabetologia. 1990 Jul;33(7):411-6. doi: 10.1007/BF00404090.
To determine whether autoantibodies to the insulin receptor may represent markers of Type 1 (insulin-dependent) diabetes, the prevalence of such antibodies was investigated in sera of 60 newly diagnosed untreated Type 1 diabetic patients. A sensitive assay, based on enzyme linked immunosorbent assay has been set up which detects antibodies to the insulin receptor irrespective of their potentially inhibiting effect on insulin binding. Moreover, this method allows easy determination of the immunoglobulin class involved in the anti-receptor activity. Among the 60 sera examined, only one was found to contain anti-insulin receptor autoantibodies (IgG class). In view of our data, we conclude that autoantibodies to the insulin receptor are infrequent findings in Type 1 diabetes of recent onset.
为了确定胰岛素受体自身抗体是否可能是1型（胰岛素依赖型）糖尿病的标志物，我们对60例新诊断的未经治疗的1型糖尿病患者血清中此类抗体的患病率进行了调查。我们建立了一种基于酶联免疫吸附测定的灵敏检测方法，该方法可检测胰岛素受体抗体，而不论其对胰岛素结合的潜在抑制作用如何。此外，该方法能够轻松确定参与抗受体活性的免疫球蛋白类别。在所检测的60份血清中，仅发现一份含有抗胰岛素受体自身抗体（IgG类）。基于我们的数据，我们得出结论，胰岛素受体自身抗体在近期发病的1型糖尿病中并不常见。
