Department of Chemistry, Columbia University, New York, NY 10027, USA.
Curr Opin Chem Biol. 2011 Dec;15(6):831-7. doi: 10.1016/j.cbpa.2011.10.005. Epub 2011 Nov 4.
Imaging contrasts other than fluorescence are highly desirable for label-free detection and interrogation of nonfluorescent molecular species inside live cells, tissues, and organisms. The recently developed stimulated Raman scattering (SRS) and stimulated emission microscopy techniques provide sensitive and specific contrast mechanisms for nonfluorescent species, by employing the light amplification aspect of stimulated radiation. Compared to their spontaneous counterparts, stimulated radiation can enhance the imaging performance significantly, making the previously 'dark' molecules observable. Here we review and summarize the underlying principles of this emerging class of molecular imaging techniques.
除荧光之外的成像对比对于在活细胞、组织和生物体内无荧光分子物种的无标记检测和询问是非常理想的。最近开发的受激拉曼散射（SRS）和受激发射显微镜技术通过利用受激辐射的光放大方面，为非荧光物种提供了敏感和特异的对比机制。与自发辐射相比，受激辐射可以显著增强成像性能，使得以前“暗”的分子变得可观察。在这里，我们回顾和总结了这一类新兴分子成像技术的基本原理。
