Fu Dan
University of Washington, Department of Chemistry, Seattle, WA 98195, United States.
Curr Opin Chem Biol. 2017 Aug;39:24-31. doi: 10.1016/j.cbpa.2017.05.002. Epub 2017 May 22.
Chemical imaging plays an increasingly important role in studying heterogeneous biological systems. It combines molecular spectroscopy with high-resolution spatial information to create quantitative images of molecular distributions. Here I summarize recent progress in technical developments and biological applications of a specific chemical imaging technique-stimulated Raman scattering (SRS) microscopy. SRS microscopy allows for a wide range of molecules - both endogenous and exogenous - to be imaged at high spatial and temporal resolution in living cells, tissues, and organisms. I will focus on developments in SRS imaging that enable detection of non-fluorescent molecules and drive new biological applications.
化学成像在研究异质生物系统中发挥着越来越重要的作用。它将分子光谱与高分辨率空间信息相结合，以创建分子分布的定量图像。在此，我总结了一种特定化学成像技术——受激拉曼散射（SRS）显微镜在技术发展和生物应用方面的最新进展。SRS显微镜能够在活细胞、组织和生物体中，以高空间和时间分辨率对多种内源性和外源性分子进行成像。我将重点关注SRS成像技术的发展，这些发展能够检测非荧光分子并推动新的生物应用。
