Department of Applied Physics, Osaka University, Suita, Osaka, Japan.
Curr Opin Chem Biol. 2013 Aug;17(4):708-15. doi: 10.1016/j.cbpa.2013.05.021. Epub 2013 Jun 15.
Raman microscopy represents an emerging class of tools for molecular imaging of live cells because of the rich information obtained by detecting molecular vibrations. Recently, several Raman imaging techniques based on the parallel detection of Raman spectra have been developed, which can achieve high spatial and temporal resolution suitable for live cell imaging. When combined with tiny Raman tags in the cellular silent region, Raman microscopy has capability to map the distribution of specific target small molecules with minimum perturbation from the tag. Here we review these recent advances in cell imaging techniques based on spontaneous Raman scattering and highlight its potential for the observation and analysis of biological functions.
拉曼显微镜是一种新兴的活细胞分子成像工具，因为它可以通过检测分子振动获得丰富的信息。最近，已经开发出了几种基于拉曼光谱并行检测的拉曼成像技术，这些技术可以实现适用于活细胞成像的高空间和时间分辨率。当与细胞静默区中的微小拉曼标签结合使用时，拉曼显微镜具有以最小的标签干扰来绘制特定靶小分子分布的能力。本文综述了基于自发拉曼散射的细胞成像技术的这些最新进展，并强调了其在观察和分析生物功能方面的潜力。
