Department of Psychology, Southern Methodist University, 6116 N. Central Expressway, Dallas, TX 75206, USA.
Physiol Behav. 2012 Feb 1;105(3):841-9. doi: 10.1016/j.physbeh.2011.10.014. Epub 2011 Oct 25.
Previous research suggests that acute stress can increase the release of immune-relevant proteins in saliva. However, no attempts have been made to examine a wider range of salivary proteins in response to stress. In this study, we identified and quantified changes in the pattern of salivary protein release in a 45 min time period following the Trier Social Stress Test (TSST) in 12 asthmatic and 13 healthy participants. Proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis. The relative protein amounts were quantified using the Image J software (NIH), and identified and characterized using mass spectroscopy. Negative affect was increased immediately after stress in both groups. The results showed that alpha amylase, cystatin S and light chain IgA were increased after the TSST and significant increases in glutathione S-transferase and prolactin inducible protein were also observed. Asthma patients showed responses similar to healthy controls, but had a tendency toward overall lower alpha amylase levels. Our findings suggest that a variety of proteins relevant to mucosal immunity are elevated following acute psychosocial stress, including glutathione S-transferase and prolactin inducible protein, which had not been characterized in this context before.
先前的研究表明，急性压力会增加唾液中与免疫相关的蛋白质的释放。然而，尚未有人尝试检测应激反应中更广泛的唾液蛋白质。在这项研究中，我们在 12 名哮喘患者和 13 名健康参与者中，在经受特里尔社会压力测试（TSST）后的 45 分钟时间内，确定并量化了唾液蛋白释放模式的变化。使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离蛋白质。使用 NIH 的 Image J 软件定量相对蛋白质含量，并使用质谱法进行鉴定和特征分析。两组的负面情绪在压力后立即增加。结果表明，α-淀粉酶、半胱氨酸蛋白酶抑制剂 S 和轻链 IgA 在 TSST 后增加，谷胱甘肽 S-转移酶和催乳素诱导蛋白的显著增加也观察到。哮喘患者的反应与健康对照组相似，但 α-淀粉酶水平总体较低。我们的研究结果表明，多种与黏膜免疫相关的蛋白质在急性心理社会压力后升高，包括谷胱甘肽 S-转移酶和催乳素诱导蛋白，以前在这种情况下尚未对其进行特征分析。
