补体引起的脂质双层分子重排：一种膜溶解的可能机制。
Molecular reorganization of lipid bilayers by complement: a possible mechanism for membranolysis.
作者信息
Esser A F, Kolb W P, Podack E R, Müller-Eberhard H J
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1410-4. doi: 10.1073/pnas.76.3.1410.
Abstract
The interaction between the membrane attack complex (MAC) of complement and flat lipid bilayers was investigated. Using spin-labeled derivatives of phospholipids and cholesterol and electron paramagnetic resonance spectroscopy, we measured the penetration of the MAC into bilayers and its influence on the order of bilayers. The MAC precursor components C5b--6, C7, C8, and C9 did not exert any measurable influence on lipid membranes. Functional C5b--7 was shown to interact strongly with the bilayer surface without deep penetration into the bilayer. Formation of C5b--8 and especially C5b--9 caused a marked change in the anisotropy of spectra from probes located within the hydrocarbon phase. The spectral changes are not caused by changes in probe rotation and, in the case of the cholesterol probes, are not due to direct probe--protein interactions. For these reasons we interpret the spectral changes to be the result of reorientation of ordered bilayer lipids effected by strong binding of phospholipids to MAC proteins.
摘要
研究了补体膜攻击复合物（MAC）与扁平脂质双层之间的相互作用。我们使用磷脂和胆固醇的自旋标记衍生物以及电子顺磁共振光谱，测量了MAC对双层的渗透及其对双层有序性的影响。MAC前体成分C5b-6、C7、C8和C9对脂质膜没有任何可测量的影响。功能性C5b-7被证明与双层表面强烈相互作用，但不会深入双层内部。C5b-8尤其是C5b-9的形成导致位于烃相内的探针光谱各向异性发生显著变化。光谱变化不是由探针旋转的变化引起的，对于胆固醇探针而言，也不是由于直接的探针-蛋白质相互作用。基于这些原因，我们将光谱变化解释为磷脂与MAC蛋白的强结合导致有序双层脂质重新定向的结果。
相似文献
1
Molecular reorganization of lipid bilayers by complement: a possible mechanism for membranolysis.补体引起的脂质双层分子重排：一种膜溶解的可能机制。
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1410-4. doi: 10.1073/pnas.76.3.1410.
2
Membrane attack complex of complement: distribution of subunits between the hydrocarbon phase of target membranes and water.补体膜攻击复合物：靶膜碳氢相和水之间亚基的分布
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4544-8. doi: 10.1073/pnas.78.7.4544.
3
Membrane attack complex of complement: generation of high-affinity phospholipid binding sites by fusion of five hydrophilic plasma proteins.补体膜攻击复合物：通过五种亲水性血浆蛋白融合产生高亲和力磷脂结合位点。
Proc Natl Acad Sci U S A. 1979 Feb;76(2):897-901. doi: 10.1073/pnas.76.2.897.
4
On the mechanism of cytolysis by complement: evidence on insertion of C5b and C7 subunits of the C5b,6,7 complex into phospholipid bilayers of erythrocyte membranes.关于补体介导细胞溶解的机制：C5b,6,7复合物中C5b和C7亚基插入红细胞膜磷脂双层的证据。
Proc Natl Acad Sci U S A. 1975 Dec;72(12):5076-80. doi: 10.1073/pnas.72.12.5076.
5
Increased ion permeability of planar lipid bilayer membranes after treatment with the C5b-9 cytolytic attack mechanism of complement.在用补体的C5b-9溶细胞攻击机制处理后，平面脂质双分子层膜的离子通透性增加。
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2852-6. doi: 10.1073/pnas.73.8.2852.
6
Cholesterol attenuates and prevents bilayer damage and breakdown in lipoperoxidized model membranes. A spin labeling EPR study.胆固醇可减轻并防止脂质过氧化模型膜中的双层损伤和破裂。一项自旋标记电子顺磁共振研究。
Biochim Biophys Acta. 2011 Sep;1808(9):2267-74. doi: 10.1016/j.bbamem.2011.04.016. Epub 2011 May 7.
7
Molecular composition of the tubular structure of the membrane attack complex of complement.补体膜攻击复合物管状结构的分子组成。
J Biol Chem. 1984 Jul 10;259(13):8641-7.
8
On domain structure and local curvature in lipid bilayers and biological membranes.关于脂质双层膜和生物膜中的结构域结构与局部曲率
Z Naturforsch C Biosci. 1977 Jul-Aug;32(7-8):581-96. doi: 10.1515/znc-1977-7-817.
9
Heterogeneous MAC Initiator and Pore Structures in a Lipid Bilayer by Phase-Plate Cryo-electron Tomography.相衬低温电子断层成像术研究脂双层中的异质 MAC 引发子和孔结构。
Cell Rep. 2016 Apr 5;15(1):1-8. doi: 10.1016/j.celrep.2016.03.002. Epub 2016 Mar 24.
10
Ultrastructure of the membrane attack complex of complement: detection of the tetramolecular C9-polymerizing complex C5b-8.补体膜攻击复合物的超微结构：四聚体C9聚合复合物C5b-8的检测
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7474-8. doi: 10.1073/pnas.79.23.7474.
引用本文的文献
1
Complement C5b-9 and Cancer: Mechanisms of Cell Damage, Cancer Counteractions, and Approaches for Intervention.补体 C5b-9 与癌症：细胞损伤的机制、癌症的拮抗作用及干预方法。
Front Immunol. 2019 Apr 10;10:752. doi: 10.3389/fimmu.2019.00752. eCollection 2019.
2
CryoEM reveals how the complement membrane attack complex ruptures lipid bilayers.冷冻电镜揭示补体膜攻击复合物如何破坏脂双层。
Nat Commun. 2018 Dec 14;9(1):5316. doi: 10.1038/s41467-018-07653-5.
3
Structural basis of complement membrane attack complex formation.补体膜攻击复合物形成的结构基础。
Nat Commun. 2016 Feb 4;7:10587. doi: 10.1038/ncomms10587.
4
T cell activation by terminal complex of complement and immune complexes.T 细胞被补体末端复合物和免疫复合物激活。
J Biol Chem. 2011 Nov 4;286(44):38627-38637. doi: 10.1074/jbc.M111.266809. Epub 2011 Sep 7.
5
Crystal structure of the MACPF domain of human complement protein C8 alpha in complex with the C8 gamma subunit.人补体蛋白C8α的MACPF结构域与C8γ亚基复合物的晶体结构。
J Mol Biol. 2008 May 30;379(2):331-42. doi: 10.1016/j.jmb.2008.03.061. Epub 2008 Apr 3.
6
Effects of temperature, time, and toxin concentration on lesion formation by the Escherichia coli hemolysin.温度、时间和毒素浓度对大肠杆菌溶血素形成损伤的影响。
Infect Immun. 1994 Oct;62(10):4124-34. doi: 10.1128/iai.62.10.4124-4134.1994.
7
Membrane attack complex of complement: a structural analysis of its assembly.补体膜攻击复合物：其组装的结构分析
J Exp Med. 1980 Feb 1;151(2):301-13. doi: 10.1084/jem.151.2.301.
8
Ultrastructure of the membrane attack complex of complement: detection of the tetramolecular C9-polymerizing complex C5b-8.补体膜攻击复合物的超微结构：四聚体C9聚合复合物C5b-8的检测
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7474-8. doi: 10.1073/pnas.79.23.7474.
9
Polymerization of the ninth component of complement (C9): formation of poly(C9) with a tubular ultrastructure resembling the membrane attack complex of complement.补体第九成分（C9）的聚合：形成具有类似于补体膜攻击复合物的管状超微结构的多聚（C9）。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):574-8. doi: 10.1073/pnas.79.2.574.
10
Permeability characteristics of complement-damaged membranes: evaluation of the membrane leak generated by the complement proteins C5b-9.补体损伤膜的通透性特征：对补体蛋白C5b - 9产生的膜渗漏的评估。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1838-42. doi: 10.1073/pnas.78.3.1838.
本文引用的文献
1
Multilayers of phospholipid bimolecular leaflets.多层磷脂双分子片层。
Nature. 1968 Nov 9;220(5167):577-8. doi: 10.1038/220577a0.
2
Release of trapped marker from liposomes by the action of purified complement components.纯化补体成分作用下脂质体中捕获标记物的释放。
Proc Natl Acad Sci U S A. 1969 Sep;64(1):290-5. doi: 10.1073/pnas.64.1.290.
3
Molecular analysis of the membrane attack mechanism of complement.补体膜攻击机制的分子分析
J Exp Med. 1972 Mar 1;135(3):549-66. doi: 10.1084/jem.135.3.549.
4
Mode of action of human C9: adsorption of multiple C9 molecules to cell-bound C8.人补体C9的作用方式：多个C9分子吸附到细胞结合的C8上。
J Immunol. 1974 Aug;113(2):479-88.
5
Phase transitions in phospholipid vesicles. Fluorescence polarization and permeability measurements concerning the effect of temperature and cholesterol.磷脂囊泡中的相变。关于温度和胆固醇影响的荧光偏振和渗透性测量。
Biochim Biophys Acta. 1973 Jul 6;311(3):330-48. doi: 10.1016/0005-2736(73)90314-3.
6
Antibody-complement interaction with lipid model membranes.抗体 - 补体与脂质模型膜的相互作用。
Biochim Biophys Acta. 1972 Feb 14;265(1):1-23. doi: 10.1016/0304-4157(72)90017-2.
7
Conformation of protein in biological membranes and a model transmembrane channel.生物膜中蛋白质的构象及一种跨膜通道模型
Ann N Y Acad Sci. 1972 Jun 20;195:108-25.
8
Structural requirements for the formation of ordered lipid multibilayers--a spin probe study.有序脂质多层膜形成的结构要求——自旋探针研究
Arch Biochem Biophys. 1973 Nov;159(1):297-311. doi: 10.1016/0003-9861(73)90456-6.
9
Studies on the terminal stages of complement lysis.补体溶解终末阶段的研究。
Immunology. 1973 Jan;24(1):135-45.
10
Bent fatty acid chains in lecithin bilayers.卵磷脂双分子层中弯曲的脂肪酸链。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1274-8. doi: 10.1073/pnas.68.6.1274.
Zotero 插件