Burgos Carmen, Carrodeguas José Alberto, Moreno Carlos, Altarriba Juan, Tarrafeta Luis, Barcelona José Antonio, López-Buesa Pascual
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Meat Sci. 2006 May;73(1):144-50. doi: 10.1016/j.meatsci.2005.11.007. Epub 2006 Jan 19.
MC4R, melanocortin-4 receptor, is involved in feed intake regulation. A mutation in a single base of MC4R, a G/A substitution in position 1426, has been linked to enhanced backfat thickness, average daily gain and daily feed intake. We present in this work a method to diagnose this mutation using real time PCR (RT-PCR) which allows rapid, cheap and reliable analysis of hundreds of samples in just 2h after DNA extraction. We have used this RT-PCR based assay to study the incidence of the mutation in several pig breeds or crosses (Iberian, Duroc, Pietrain, Large White, Large White×Pietrain) and wild boars. IGF2, insuline like growth factor 2, a gene with well demonstrated effects on carcass composition, of all these animals has also been analyzed and we show, using linkage disequilibrium analysis that both genes are independent. The implications of our results for pig selection toward fatty or lean carcasses are discussed.
促黑素皮质素4受体（MC4R）参与采食量调节。MC4R的一个单碱基突变，即第1426位的G/A替换，与背膘厚度增加、平均日增重和日采食量有关。在这项工作中，我们提出了一种使用实时聚合酶链反应（RT-PCR）诊断该突变的方法，该方法能够在DNA提取后仅2小时内对数百个样本进行快速、廉价且可靠的分析。我们已使用这种基于RT-PCR的检测方法研究了几个猪品种或杂交品种（伊比利亚猪、杜洛克猪、皮特兰猪、大白猪、大白猪×皮特兰猪）以及野猪中该突变的发生率。还分析了所有这些动物中对胴体组成有明确影响的胰岛素样生长因子2（IGF2）基因，并且我们通过连锁不平衡分析表明这两个基因是独立的。讨论了我们的结果对选择肥胴体或瘦胴体猪的意义。
