Rius M A, García-Regueiro J A
IRTA Unitat Quı́mica Alimentària, Centre Tecnologia de la Carn, Granja Camps i Armet, 17121 Monells, Spain.
Meat Sci. 2001 Nov;59(3):285-91. doi: 10.1016/s0309-1740(01)00081-x.
A HPLC-NP (normal phase-high performance liquid chromatography) method for determining the concentration of skatole and indole in Longissimus dorsi samples is described. Lipids containing skatole and indole were extracted in chloroform:methanol (2:1) at room temperature and dehydrated by liquid-liquid extraction with an aqueous solution saturated with 10% of sodium chloride. The organic phase was evaporated to dryness and redissolved in 10 ml of hexane:2-propanol (92:8). Indolic compounds were separated on a Hypersil aminopropylsilica column (5 μm) (250×4.6 mm i.d.). The mobile phase was hexane:2-propanol (92:8) and detection was by fluorescence (excitation at 280 nm and emission at 360 nm). Linearity was found in the range of 0.05-0.4 μg/g and the coefficient of correlation was above 0.99 for both compounds. The within day (n=5) variation was at 0.05, 0.2 and 0.4 μg/g and the CV (coefficient of variation) values for relative areas determined at these concentrations were less than 13%. This method was used to compare the concentrations of skatole and indole in different samples: L. dorsi muscle, the fat covering the L. dorsi and subcutaneous fat. A correlation was observed between the concentration of indole and skatole in the back fat and fat covering the L. dorsi samples (P<0.001, r=0.99). No significant correlation was obtained in L. dorsi samples, between skatole and indole levels. In spite of the correlation shown between skatole and indole concentrations in the back fat and L. dorsi samples, the mean concentrations of these compounds were significantly higher (P<0.05) in the back fat samples.
本文描述了一种用于测定背最长肌样品中粪臭素和吲哚浓度的高效液相色谱-正相（HPLC-NP）法。含粪臭素和吲哚的脂质在室温下用氯仿：甲醇（2:1）提取，并用10%氯化钠饱和水溶液进行液-液萃取脱水。有机相蒸发至干，再溶解于10 ml己烷：2-丙醇（92:8）中。吲哚类化合物在Hypersil氨丙基硅胶柱（5μm）（250×4.6 mm内径）上分离。流动相为己烷：2-丙醇（92:8），采用荧光检测（激发波长280 nm，发射波长360 nm）。两种化合物在0.05 - 0.4μg/g范围内呈线性，相关系数均高于0.99。日内（n = 5）变异在0.05、0.2和0.4μg/g水平，这些浓度下相对面积的变异系数（CV）值小于13%。该方法用于比较不同样品中粪臭素和吲哚的浓度：背最长肌、覆盖背最长肌的脂肪和皮下脂肪。在背膘和覆盖背最长肌的脂肪样品中，吲哚和粪臭素浓度之间存在相关性（P<0.001，r = 0.99）。在背最长肌样品中，粪臭素和吲哚水平之间未获得显著相关性。尽管在背膘和背最长肌样品中粪臭素和吲哚浓度之间显示出相关性，但这些化合物在背膘样品中的平均浓度显著更高（P<0.05）。
