Centro de Biotecnologia Molecular Estrutural, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
FEBS Lett. 2011 Dec 15;585(24):3868-73. doi: 10.1016/j.febslet.2011.10.043. Epub 2011 Nov 3.
We describe the purification, crystallization and structure for the GTP-binding domain of human septin 7 (SEPT7G). We show that it forms filaments within the crystal lattice which employ both the G and NC interfaces, similar to those seen in the hetero-filament of SEPT2/6/7. The NC interface is considered promiscuous as it is absent from the hetero-filament. Such promiscuity could provide the potential for permuting monomers along a filament in order to generate diversity in hetero-polymers. On the other hand, our results suggest that the G and NC interfaces may be necessary but insufficient for determining correct hetero-filament assembly.
我们描述了人源 septin 7（SEPT7G）的 GTP 结合域的纯化、结晶和结构。我们表明，它在晶格内形成纤维，既采用 G 接口又采用 NC 接口，类似于 SEPT2/6/7 异源二聚体中看到的纤维。NC 接口被认为是杂乱无章的，因为它不存在于异源二聚体中。这种杂乱无章可能为沿着纤维交换单体提供了潜力，以便在异源聚合物中产生多样性。另一方面，我们的结果表明，G 和 NC 接口可能是确定正确的异源二聚体组装所必需的，但不是充分的。
