Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Solna, Sweden.
Arch Dermatol Res. 2012 Jan;304(1):15-22. doi: 10.1007/s00403-011-1189-x. Epub 2011 Nov 8.
Microorganisms have been implicated in the pathogenesis of psoriasis. Previous studies of psoriasis and normal skin have used swabs from the surface rather than skin biopsies. In this study, biopsies were taken from 10 patients with psoriasis and 12 control subjects from unmatched sites. Samples were analysed with massive parallel pyrosequencing on the 454 platform targeting the 16S rRNA gene and the variable regions V3-V4. The samples grouped into 19 phyla, 265 taxon and 652 operational units (OTUs) at 97% identity. A cut-off abundance level was set at 1%. The three most common phyla in both normal and psoriasis skin were Firmicutes (39% psoriasis, 43% normal skin), Proteobacteria (38% psoriasis, 27% normal skin) and Actinobacteria (5% psoriasis, 16% normal skin, p = 0.034). In trunk skin, Proteobacteria were present at significantly higher levels in psoriasis compared to controls (52 vs. 32%, p = 0.0113). The commonest genera were Streptococci in both psoriasis (32%) and normal skin (26%). Staphylococci were less common in psoriasis (5%) than in controls (16%), as were Propionibacteria (psoriasis 0.0001669%, controls 0.0254%). Both Staphylococci and Propionibacteria were significantly lower in psoriasis versus control limb skin (p = 0.051, 0.046, respectively). This study has shown some differences in microbiota between psoriasis and normal skin. Whether these are of primary aetiological significance, or secondary to the altered skin of psoriasis remains to be determined.
微生物已被牵涉到银屑病的发病机制中。以前对银屑病和正常皮肤的研究使用的是来自表面的拭子，而不是皮肤活检。在这项研究中，从 10 名银屑病患者和 12 名来自非匹配部位的对照者中采集活检样本。使用 454 平台上针对 16S rRNA 基因和可变区 V3-V4 的大规模平行焦磷酸测序技术对样本进行分析。样本分为 19 个门、265 个分类群和 652 个操作单元（OTUs），相似度为 97%。设定丰度水平的截止值为 1%。在正常和银屑病皮肤中最常见的三个门是厚壁菌门（39%银屑病，43%正常皮肤）、变形菌门（38%银屑病，27%正常皮肤）和放线菌门（5%银屑病，16%正常皮肤，p=0.034）。在躯干皮肤中，与对照组相比，银屑病患者中变形菌门的存在水平明显更高（52%比 32%，p=0.0113）。最常见的属在银屑病和正常皮肤中均为链球菌（银屑病 32%，正常皮肤 26%）。与对照组相比，葡萄球菌在银屑病中较少见（5%比 16%），而丙酸杆菌也是如此（银屑病 0.0001669%，对照组 0.0254%）。与对照组相比，葡萄球菌和丙酸杆菌在银屑病皮肤中均显著减少（p=0.051，0.046）。本研究显示了银屑病与正常皮肤之间的一些微生物群落差异。这些差异是原发性病因学意义，还是银屑病改变的皮肤的继发性结果，仍有待确定。
