School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.
Langmuir. 2012 Jan 10;28(1):883-8. doi: 10.1021/la203704j. Epub 2011 Nov 30.
In this paper, we report on the Ag nanoparticle-containing hybrid poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM-co-PAA) microgels with pH- and thermoresponsive metal-enhanced fluorescence (MEF). The hybrid microgels were prepared by in situ reducing Ag salts to Ag nanoparticles in the PNIPAM-co-PAA microgels. According to the interaction distance-dependent nature of MEF effects, we can realize a controllable MEF effect by adjusting the average interaction distance between fluorophores and Ag nanoparticles due to the good stimuli-responsive swelling-shrinking behavior of the hybrid microgels. The results show that MEF effect can be well tuned in the pH region 2-12 as well as the temperature region of 20-50 °C. By this method, an enhanced fluorescence detection can possibly be manipulated by adjusting external stimuli such as pH and temperature.
本文报道了一种载银纳米粒子的聚（N-异丙基丙烯酰胺-共-丙烯酸）（PNIPAM-co-PAA）杂化温敏和 pH 响应型金属增强荧光（MEF）微凝胶。该杂化微凝胶是通过在 PNIPAM-co-PAA 微凝胶中原位还原 Ag 盐得到的 Ag 纳米粒子。根据 MEF 效应的相互作用距离依赖性，我们可以通过调节荧光团和 Ag 纳米粒子之间的平均相互作用距离来实现可控的 MEF 效应，这是由于杂化微凝胶具有良好的刺激响应性溶胀-收缩行为。结果表明，MEF 效应可以在 pH 2-12 以及 20-50°C 的温度范围内得到很好的调节。通过这种方法，通过调节 pH 和温度等外部刺激，可能可以对增强荧光检测进行操纵。
