Hacettepe University, Faculty of Pharmacy, Department of Pharmacognosy, TR-06100 Ankara, Turkey.
Food Chem Toxicol. 2012 Feb;50(2):141-6. doi: 10.1016/j.fct.2011.10.061. Epub 2011 Oct 31.
Quercus species are used as antidiarrheic, for the treatment of hemorrhoid, oral and anal mucosa inflammation. These tree species have been of interest to researchers because of their usage in folk medicine, consumption as food, beverage and especially usage of oak woods for construction in wine barrels. The DPPH, SO and NO radical scavenging activities, protective effect against H2O2 induced cytotoxicity as well as their cytotoxic activity against Hep-2 human larynx epidermoid carcinoma cell line of the MeOH and water extracts of the barks of Quercus cerris var. cerris, Quercusmacranthera subsp. syspirensis and Quercus aucheri were investigated for the first time. Total phenolic content of the extracts was also evaluated by Folin-Ciocalteu method. Results demonstrated that the extracts showed strong radical scavenging activity comparable to those of standard compounds. Extracts also showed good protective effect against H2O2 induced cytotoxicity on human erythrocytes comparing to ascorbic acid. On the other hand, while each extract showed dose dependent cytotoxic activity, MeOH extract of Q.macranthera subsp. syspirensis showed the strongest cytotoxicity against the tested cell line. Taken together, the results showed that Quercus species may be a promising alternative to synthetic substances as natural compound with high antioxidant and antiproliferative activities.
栎属物种被用作止泻药，用于治疗痔疮、口腔和肛门黏膜炎症。由于这些树种在民间医学中的应用、作为食物和饮料的消费，特别是栎木在葡萄酒桶中的建筑用途，它们一直引起研究人员的兴趣。本研究首次对栓皮栎、麻栎宽叶变种和槲栎的树皮的甲醇和水提取物的 DPPH、SO 和 NO 自由基清除活性、对 H2O2 诱导的人红细胞细胞毒性的保护作用以及对 Hep-2 人喉表皮样癌细胞系的细胞毒性进行了研究。还通过福林-考尔特尔法评估了提取物的总酚含量。结果表明，提取物表现出与标准化合物相当的强自由基清除活性。与抗坏血酸相比，提取物对 H2O2 诱导的人红细胞毒性也表现出良好的保护作用。另一方面，虽然每种提取物均表现出剂量依赖性的细胞毒性，但麻栎宽叶变种的甲醇提取物对测试细胞系表现出最强的细胞毒性。总之，研究结果表明，栎属植物可能是一种很有前途的替代合成物质的天然化合物，具有高抗氧化和抗增殖活性。
