Department of Pharmacognosy, Hacettepe University, 06100 Ankara, Turkey.
Pharm Biol. 2011 Nov;49(11):1150-7. doi: 10.3109/13880209.2011.575790. Epub 2011 May 20.
The genus Veronica L. (Plantaginaceae) is represented by 79 species, 26 of which are endemic in Turkey. Some Veronica species are used for the treatment of different inflammatory diseases and cancer in traditional medicine. In addition, chemotaxonomy of the genus is important for the reclassification of the family Plantaginaceae after different phylogenetic studies.
Veronica cuneifolia subsp. cuneifolia D. Don and V. cymbalaria Bodard were studied from the view point of iridoid glucosides which are known as chemotaxonomical markers for this genus. Radical scavenging and cytotoxic activities of the extracts were also determined in this study.
Major compounds, isolated from iridoid fractions of V. cuneifolia subsp. cuneifolia were used as the standard compounds for HPLC after determination of their structures, and investigated for their presence in iridoid fractions of V. cymbalaria. Additionally, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and SO radical scavenging and cytotoxic activities against three cancer and a noncancerous cell lines of both extract were also tested using the MTT method.
While aucubin, catalpol, verproside, amphicoside, verminoside, and veronicoside were obtained from V. cuneifolia subsp. cuneifolia, two more iridoid glucosides, 6-O-veratroylcatalposide and 6-O-isovanilloylcatalpol, were isolated from V. cymbalaria. Comparing both species, V. cuneifolia subsp. cuneifolia showed stronger radical scavenging and cytotoxic activities than V. cymbalaria.
Our results demonstrated that the iridoid contents of both species were very close to each other confirming to the chemotaxonomic studies on Veronica species and their different bioactivity range make the plants interesting from the view point of natural drug discovery research.
婆婆纳属（玄参科）有 79 种，其中 26 种为土耳其特有种。一些婆婆纳种在传统医学中用于治疗不同的炎症性疾病和癌症。此外，在不同的系统发育研究之后，该属的化学分类学对于玄参科的重新分类非常重要。
从作为该属化学分类学标记的环烯醚萜苷的角度研究了 Veronica cuneifolia subsp. cuneifolia D. Don 和 V. cymbalaria Bodard。在这项研究中还测定了提取物的自由基清除和细胞毒性活性。
从 Veronica cuneifolia subsp. cuneifolia 的环烯醚萜部分分离出的主要化合物在确定其结构后被用作 HPLC 的标准化合物，并研究了它们在 V. cymbalaria 环烯醚萜部分的存在情况。此外，还使用 MTT 法测试了 2,2-二苯基-1-苦基肼（DPPH）和 SO 自由基清除以及对两种提取物的三种癌细胞和一种非癌细胞系的细胞毒性活性。
从 Veronica cuneifolia subsp. cuneifolia 中得到了梓醇、毛蕊花糖苷、獐牙菜苦苷、獐牙菜苷、苦柯脂苷和 Veronicaoside，而从 V. cymbalaria 中分离出了另外两种环烯醚萜苷，6-O-香草酰基梓醇和 6-O-异香草酰基梓醇。比较这两个物种，Veronica cuneifolia subsp. cuneifolia 比 V. cymbalaria 显示出更强的自由基清除和细胞毒性活性。
我们的结果表明，这两个物种的环烯醚萜含量非常接近，证实了对 Veronica 种的化学分类学研究，并且它们不同的生物活性范围使这些植物从天然药物发现研究的角度变得有趣。
