Department of Environmental Engineering, Technical University of Denmark, DK-2800, Kgs Lyngby, Denmark.
Biotechnol Bioeng. 2012 Apr;109(4):1088-94. doi: 10.1002/bit.24360. Epub 2011 Nov 21.
The possibility of converting hydrogen to methane and simultaneous upgrading of biogas was investigated in both batch tests and fully mixed biogas reactor, simultaneously fed with manure and hydrogen. Batch experiments showed that hydrogen could be converted to methane by hydrogenotrophic methanogenesis with conversion of more than 90% of the consumed hydrogen to methane. The hydrogen consumption rates were affected by both P(H₂) (hydrogen partial pressure) and mixing intensity. Inhibition of propionate and butyrate degradation by hydrogen (1 atm) was only observed under high mixing intensity (shaking speed 300 rpm). Continuous addition of hydrogen (flow rate of 28.6 mL/(L/h)) to an anaerobic reactor fed with manure, showed that more than 80% of the hydrogen was utilized. The propionate and butyrate level in the reactor was not significantly affected by the hydrogen addition. The methane production rate of the reactor with H₂ addition was 22% higher, compared to the control reactor only fed with manure. The CO₂ content in the produced biogas was only 15%, while it was 38% in the control reactor. However, the addition of hydrogen resulted in increase of pH (from 8.0 to 8.3) due to the consumption of bicarbonate, which subsequently caused slight inhibition of methanogenesis.
研究了在分批试验和完全混合沼气反应器中，同时用粪便和氢气进料，将氢气转化为甲烷并同时对沼气进行升级的可能性。分批实验表明，氢气可以通过氢营养型产甲烷作用转化为甲烷，超过 90%的消耗氢气转化为甲烷。氢气消耗速率受 P(H₂)（氢气分压）和混合强度的影响。仅在高混合强度（搅拌速度为 300rpm）下观察到氢气（1atm）对丙酸和丁酸降解的抑制作用。连续向仅用粪便进料的厌氧反应器中添加氢气（流速为 28.6mL/（L/h）），表明超过 80%的氢气得到了利用。氢气添加对反应器中丙酸和丁酸的水平没有显著影响。与仅用粪便进料的对照反应器相比，添加氢气的反应器的甲烷生成率提高了 22%。产生的沼气中 CO₂的含量仅为 15%，而对照反应器中的含量为 38%。然而，由于碳酸氢盐的消耗，氢气的添加导致 pH 值（从 8.0 增加到 8.3）升高，这随后导致产甲烷作用受到轻微抑制。
