Minagawa H, Kobayashi H, Yoshida H, Teranishi M, Morikawa A, Abe S, Oshima H, Mizuno D I
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., Japan.
Br J Cancer. 1990 Sep;62(3):372-5. doi: 10.1038/bjc.1990.300.
Intratumoral induction of tumour necrosis factor (TNF) by administration of Bordetella pertussis vaccine (BPV) as compared with that by the agent OK-432 was investigated in mice. Two hours after such administration tumour tissues tested were resected from the mice, homogenised, and the TNF activities in the homogenate were assayed using a L-929 fibroblast assay. Intravenous injection of BPV into mice bearing the MM46 carcinoma resulted in a greater concentration of TNF in the tumour homogenate than in the serum. With OK-432, however, there was a greater concentration of TNF in the serum than in the tumour homogenates. A high level of intratumoral TNF induction by BPV was also observed in mice bearing Meth A fibrosarcoma or Lewis lung carcinoma. The therapeutic effect against the Meth A fibrosarcoma was in parallel with the intratumoral TNF activity. Intratumoral TNF activity is therefore believed to be a good index of therapeutic effect.
在小鼠中研究了通过给予百日咳博德特氏菌疫苗（BPV）与通过OK-432制剂在肿瘤内诱导肿瘤坏死因子（TNF）的情况。给药两小时后，从小鼠身上切除受试肿瘤组织，进行匀浆，并使用L-929成纤维细胞分析法测定匀浆中的TNF活性。给携带MM46癌的小鼠静脉注射BPV后，肿瘤匀浆中TNF的浓度高于血清中的浓度。然而，使用OK-432时，血清中TNF的浓度高于肿瘤匀浆中的浓度。在携带Meth A纤维肉瘤或Lewis肺癌的小鼠中也观察到BPV在肿瘤内诱导出高水平的TNF。对Meth A纤维肉瘤的治疗效果与肿瘤内TNF活性平行。因此，肿瘤内TNF活性被认为是治疗效果的良好指标。
