Hirata S
Department of Obstetrics and Gynecology, Teikyo University School of Medicine, Tokyo.
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Nov;42(11):1477-83.
The rabbit ovarian carcinoma model was prepared by injecting VX-2 carcinoma into the ovary and the effects of endogenous Tumor Necrosis Factor (TNF)-induction therapy in combination with chemotherapy were studied. In this model, carcinoma metastasizes easily to the abdominal cavity, lung and liver, and resists treatment. Endogenous TNF was induced by a series of injections of OK-432 0.3KE and OK-432 3KE at 3 hour intervals (OK-OK group), or 1 x 10(10) cells of Bordetella pertussis vaccine (BPV group). Another group were sequential given a series of injections of CDDP (2mg/kg) intraperitoneally and OK-432 intravenously (an OK-OK and CDDP group). Higher activity of endogenous TNF was induced by BPV in comparison with OK-OK plus OK-OK and CDDP. Obvious hemorrhagic necrosis was observed in the implanted ovary in the BPV group. Compared with the control group, pulmonary metastasis was seen in the OK-OK and OK-OK plus CDDP groups (partial remission). These results show that the endogenous TNF induction therapy could be expected to be an effective antitumor therapy for advanced ovarian cancer.
通过向兔卵巢内注射VX - 2癌来制备兔卵巢癌模型，并研究内源性肿瘤坏死因子（TNF）诱导疗法联合化疗的效果。在该模型中，癌易于转移至腹腔、肺和肝脏，且对治疗有抵抗性。通过每隔3小时注射一系列0.3KE和3KE的OK - 432（OK - OK组）或1×10(10)个百日咳博德特氏菌疫苗细胞（BPV组）来诱导内源性TNF。另一组先腹腔内注射一系列顺铂（2mg/kg），然后静脉注射OK - 432（OK - OK加顺铂组）。与OK - OK加OK - OK和顺铂相比，BPV诱导的内源性TNF活性更高。在BPV组的植入卵巢中观察到明显的出血性坏死。与对照组相比，OK - OK组和OK - OK加顺铂组出现肺转移（部分缓解）。这些结果表明，内源性TNF诱导疗法有望成为晚期卵巢癌的一种有效抗肿瘤疗法。
