浓溶液中血红蛋白A的小角中子散射研究
Small angle neutron scattering studies of HbA in concentrated solutions.
作者信息
Krueger S, Chen S H, Hofrichter J, Nossal R
机构信息
University of Maryland, College Park 20742.
出版信息
Biophys J. 1990 Sep;58(3):745-57. doi: 10.1016/S0006-3495(90)82417-6.
Abstract
Differential cross-sections for neutrons scattered by normal human hemoglobin have been determined over the range of concentrations from 2 to approximately 35 weight percent. Data are compared with structure factors calculated from models of monodisperse hard spheres interacting through a screened Coulomb potential. Good agreement is noted when the volume fraction eta is adjusted during multivariate fitting of data, but the fitted value of eta is always lower than expected from the known Hb concentration of the samples. Calculations of cross-sections for polydisperse scatterers suggest that the samples may contain oligomers of the fundamental tetrameric Hb molecule.
摘要
已测定了浓度范围为2至约35重量百分比的正常人血红蛋白散射中子的微分截面。将数据与通过屏蔽库仑势相互作用的单分散硬球模型计算出的结构因子进行了比较。当在数据的多变量拟合过程中调整体积分数η时，发现吻合良好，但η的拟合值始终低于根据样品已知血红蛋白浓度预期的值。多分散散射体的截面计算表明，样品可能包含基本四聚体血红蛋白分子的寡聚体。
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