利用中子光谱学研究红细胞中的大分子动力学。
Macromolecular dynamics in red blood cells investigated using neutron spectroscopy.
机构信息
Research Centre Jülich, , 52425 Jülich, Germany.
出版信息
J R Soc Interface. 2011 Apr 6;8(57):590-600. doi: 10.1098/rsif.2010.0306. Epub 2010 Aug 25.
Abstract
We present neutron scattering measurements on the dynamics of haemoglobin (Hb) in human red blood cells (RBCs) in vivo. Global and internal Hb dynamics were measured in the ps to ns time and Å length scales using quasi-elastic neutron backscattering spectroscopy. We observed the cross over from global Hb short-time to long-time self-diffusion. Both short- and long-time diffusion coefficients agree quantitatively with predicted values from the hydrodynamic theory of non-charged hard-sphere suspensions when a bound water fraction of around 0.23 gram H(2)O per gram Hb is taken into account. The higher amount of water in the cells facilitates internal protein fluctuations in the ps time scale when compared with fully hydrated Hb powder. Slower internal dynamics of Hb in RBCs in the ns time range were found to be rather similar to results obtained with fully hydrated protein powders, solutions and Escherichia coli cells.
摘要
我们展示了在体人红细胞（RBC）中血红蛋白（Hb）动力学的中子散射测量结果。使用准弹性中子背散射光谱法，在皮秒到纳秒时间和埃长度范围内测量了 Hb 的整体和内部动力学。我们观察到 Hb 整体从短时间到长时间自扩散的转变。当考虑到每克 Hb 结合约 0.23 克 H2O 的结合水分数时，短时间和长时间扩散系数与无电荷硬球悬浮液的流体力学理论的预测值定量一致。与完全水合的 Hb 粉末相比，细胞中更多的水有助于在皮秒时间尺度上发生内部蛋白质波动。在纳秒时间范围内，RBC 中 Hb 的较慢内部动力学与使用完全水合蛋白粉末、溶液和大肠杆菌细胞获得的结果相当相似。
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