Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; E-Mails:
Int J Mol Sci. 2011;12(10):6743-8. doi: 10.3390/ijms12106743. Epub 2011 Oct 12.
Luculia gratissima (Wall.) Sweet (Rubiaceae) is a perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar. The plant is a distylous species with reciprocally placed stigmas and anthers in each floral morph. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing (FIASCO) repeats protocol, 19 primer sets were identified in two wild populations. Of these primers, 10 displayed polymorphisms and nine were monomorphic. The number of alleles per locus ranged from two to five, values for observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.289 to 0.760, with averages of 0.303 and 0.555, respectively. These microsatellite loci will facilitate further studies on breeding system, gene flow patterns, and population structure of L. gratissima and its allied species.
厚叶桂（Wall.）Sweet（茜草科）是一种多年生灌木，分布在中国青藏高原东南部和尼泊尔、缅甸的相邻地区。该植物是一种异型花柱植物，在每个花形态中柱头和花药相互放置。通过使用序列中包含（FIASCO）重复的快速扩增片段长度多态性（AFLP）（Fast Isolation by Amplified Fragment Length Polymorphism，FIASCO）重复协议，在两个野生种群中鉴定出 19 个引物对。在这些引物中，有 10 个显示多态性，9 个为单态性。每个位点的等位基因数从 2 到 5 不等，观察到和预期的杂合度值从 0.000 到 1.000 和从 0.289 到 0.760 不等，平均值分别为 0.303 和 0.555。这些微卫星位点将有助于进一步研究厚叶桂及其近缘种的繁殖系统、基因流模式和种群结构。
