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通过扫描激光光学断层扫描仪检测散射光对植入物上的生物膜进行三维成像。

3D imaging of biofilms on implants by detection of scattered light with a scanning laser optical tomograph.

作者信息

Heidrich Marko, Kühnel Mark P, Kellner Manuela, Lorbeer Raoul-Amadeus, Lange Tineke, Winkel Andreas, Stiesch Meike, Meyer Heiko, Heisterkamp Alexander

出版信息

Biomed Opt Express. 2011 Nov 1;2(11):2982-94. doi: 10.1364/BOE.2.002982. Epub 2011 Oct 3.

DOI:10.1364/BOE.2.002982
PMID:22076261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3207369/
Abstract

Biofilms - communities of microorganisms attached to surfaces - are a constant threat for long-term success in modern implantology. The application of laser scanning microscopy (LSM) has increased the knowledge about microscopic properties of biofilms, whereas a 3D imaging technique for the large scale visualization of bacterial growth and migration on curved and non-transparent surfaces is not realized so far.Towards this goal, we built a scanning laser optical tomography (SLOT) setup detecting scattered laser light to image biofilm on dental implant surfaces. SLOT enables the visualization of living biofilms in 3D by detecting the wavelength-dependent absorption of non-fluorescent stains like e.g. reduced triphenyltetrazolium chloride (TTC) accumulated within metabolically active bacterial cells. Thus, the presented system allows the large scale investigation of vital biofilm structure and in vitro development on cylindrical and non-transparent objects without the need for fluorescent vital staining. We suggest SLOT to be a valuable tool for the structural and volumetric investigation of biofilm formation on implants with sizes up to several millimeters.

摘要

生物膜——附着于表面的微生物群落——是现代植入术长期成功的持续威胁。激光扫描显微镜（LSM）的应用增加了我们对生物膜微观特性的了解，然而，一种用于在弯曲和不透明表面大规模可视化细菌生长和迁移的三维成像技术目前尚未实现。为了实现这一目标，我们构建了一种扫描激光光学断层扫描（SLOT）装置，通过检测散射激光来对牙科植入物表面的生物膜进行成像。SLOT通过检测非荧光染料（如代谢活跃细菌细胞内积累的还原型氯化三苯基四氮唑（TTC））的波长依赖性吸收，能够以三维方式可视化活生物膜。因此，所展示的系统允许对圆柱形和不透明物体上的重要生物膜结构和体外发育进行大规模研究，而无需进行荧光活体染色。我们认为SLOT是一种有价值的工具，可用于对尺寸达数毫米的植入物上生物膜形成进行结构和体积研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/d0c62db3c015/boe-2-11-2982-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/c174dc4ab355/boe-2-11-2982-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/94ea693014d0/boe-2-11-2982-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/5470fa46887c/boe-2-11-2982-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/cb3cb409aa10/boe-2-11-2982-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/0355c023775c/boe-2-11-2982-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/cd9b419bf004/boe-2-11-2982-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/d0c62db3c015/boe-2-11-2982-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/c174dc4ab355/boe-2-11-2982-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/94ea693014d0/boe-2-11-2982-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/5470fa46887c/boe-2-11-2982-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/cb3cb409aa10/boe-2-11-2982-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/0355c023775c/boe-2-11-2982-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/cd9b419bf004/boe-2-11-2982-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7603/3207369/d0c62db3c015/boe-2-11-2982-g007.jpg

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