Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Crete, Greece.
PLoS One. 2011 Apr 29;6(4):e18963. doi: 10.1371/journal.pone.0018963.
We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms.
我们描述了一种多功能的光学投影层析成像系统，可用于快速对活体微观标本进行三维成像。我们的层析成像装置消除了传统共聚焦显微镜中光学切片在 xy 和 z 方向上产生的强烈不对称分辨率。它允许对活体透明无脊椎动物（如秀丽隐杆线虫）进行稳健的高分辨率荧光和吸收成像。与当前可用的方法相比，该系统在对动态发育过程和动物衰老进行成像时具有明显的优势；它可以以单细胞分辨率监测时空基因表达和解剖结构的改变，它同时利用荧光和吸收作为对比度的来源，并且易于适应一系列小型模式生物。
