Tichauer Kenneth M, Holt Robert W, El-Ghussein Fadi, Zhu Qun, Dehghani Hamid, Leblond Frederic, Pogue Brian W
Biomed Opt Express. 2011 Nov 1;2(11):3021-36. doi: 10.1364/BOE.2.003021. Epub 2011 Oct 5.
In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.
在本研究中，概述了用于非接触式、时间相关单光子计数小动物光学断层扫描系统的几个关键优化步骤，该系统同时采集荧光和透射率数据。展示了该系统用于体内时域图像重建，说明了单光子计数的灵敏度以及准确处理数据所需的校准步骤。特别地，在时域荧光断层扫描的背景下介绍了激光时间和幅度参考、探测器和滤波器校准以及合适的仪器响应函数的采集，并描述了一个全自动工作流程。初步的体模时域重建图像证明了基于多个时间门信号的荧光断层扫描工作流程的保真度。
